Multiple Shoots Induction and Rapid Propagation of Lonicera Edulis

DOI

10.2991/iea-15.2015.173How to use a DOI?

Keywords

lonicera edulis; multiple shoots; tissue culture; rapid propagation

Abstract

Sprouts being explants, the factors affecting in vitro culture and plant regeneration of L. edulis were optimized by orthogonal design and tissue cultured seedlings were obtained on culture media with different concentrations of hormones. The results showed that the optimum compositions for primary culture, subculture and rooting culture were MS+6-BA 1.0 mg/L + IBA 0.2 mg/L, MS+6-BA 0.5 mg/L +IBA 0.3 mg/L +KT 1.5 mg/L and 1/4MS+IBA 1.5 mg/L. The differentiation rate, proliferation coefficient and rooting rate on these media were up to 100%, 8.7, and 96.7% respectively. The combination of 6-BA, IBA and KT was effective in the number of multiple shoots of L. edulis in subculture. The micro-propagation technology introduced to cultivate the tissue cultured seedlings proved to meet the need of rapid propagation of plantlets and could be applied in mass propagation.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Qi Qige
AU  - Guo Chunyan
AU  - Zhang Qichang
PY  - 2015/09
DA  - 2015/09
TI  - Multiple Shoots Induction and Rapid Propagation of Lonicera Edulis
BT  - Proceedings of the AASRI International Conference on Industrial Electronics and Applications (2015)
PB  - Atlantis Press
SP  - 695
EP  - 699
SN  - 2352-5401
UR  - https://doi.org/10.2991/iea-15.2015.173
DO  - 10.2991/iea-15.2015.173
ID  - Qige2015/09
ER  -