Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)

Naturally Inhibited Yacon Tubers Prebiotic Syrup Increases Humoral and Cellular Immunity

Authors
Leny Yuanita1, *, Prima R. Wikandari2, Dewi Prastiwi2, Raymond I. Avandi3, Wahyu B. Sabtiawan4, Erlix R. Purnama5
1Departement of Chemistry, Universitas Negeri Surabaya, Indonesia
2Departement of Accounting, Universitas Negeri Surabaya, Indonesia
3Departement of Sport Science, Universitas Negeri Surabaya, Indonesia
4Departement of Natural Science, Universitas Negeri Surabaya, Indonesia
5Departement of Biology, Universitas Negeri Surabaya, Indonesia
*Corresponding author. Email: wahyusabtiawan@unesa.ac.id
Corresponding Author
Leny Yuanita
Available Online 16 December 2021.
DOI
https://doi.org/10.2991/aer.k.211215.089How to use a DOI?
Keywords
Prebiotic syrup; Humoral; Cellular immunity
Abstract

The aim of the study: to obtain yacon prebiotic syrup that can increase cellular and humoral immunity. Sixty of Rattus norvegicus aged about 40 days and weighing about 100-150 grams divided into 4 groups. Commercial FOS supplement group, non-inhibited yacon syrup, inhibited yacon syrup (FOS content ± 20% w/w diet), and water as a negative control. Induction of 2% sheep red blood cell antigen via intraperitoneal injection. Bioactive assay of FOS and chlorogenic acid in yacon syrup using HPLC and voltametry. CD4+/CD8+ cellular immunity was tested by floxytometry, while IgM and IgG humoral immunity by antibody titrant hemagglutination method. Data were analyzed statistically parametric and non parametric (α = 5%). The results showed: 1) inhibited yacon syrup had higher content of bioactive FOS and chlorogenic acid than non-inhibited yacon syrup. 2) The use of natural inhibitors is effective in maintaining the bioactive content of yacon tubers. 3) Supplementation of inhibited yacon syrup more effective in increasing cellular (CD4+/CD8+) and humoral (IgM and IgG) immunity against the control group than the non-inhibited yacon syrup.

Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)
Series
Advances in Engineering Research
Publication Date
16 December 2021
ISBN
978-94-6239-486-5
ISSN
2352-5401
DOI
https://doi.org/10.2991/aer.k.211215.089How to use a DOI?
Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Leny Yuanita
AU  - Prima R. Wikandari
AU  - Dewi Prastiwi
AU  - Raymond I. Avandi
AU  - Wahyu B. Sabtiawan
AU  - Erlix R. Purnama
PY  - 2021
DA  - 2021/12/16
TI  - Naturally Inhibited Yacon Tubers Prebiotic Syrup Increases Humoral and Cellular Immunity
BT  - Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)
PB  - Atlantis Press
SP  - 526
EP  - 531
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.211215.089
DO  - https://doi.org/10.2991/aer.k.211215.089
ID  - Yuanita2021
ER  -