Isolation and Identification of Endophytic Bacteria Related to Plant Nutrient Level in Coal Mining Site from East Kalimantan Indonesia
- 10.2991/aer.k.211215.082How to use a DOI?
- Coal mining area; Identification of endophytic microorganisms; East Borneo
East Kalimantan has a very wide area for coal mining. However, not all coal mining companies carry out land reclamation before coal mine was abandoned. This marginal land is very potential to be utilized as productive land, although efforts must be done to minimize the main limiting factor in coal mining site, namely low soil fertility by utilizing endophytic bacteria found in ex-coal mining sites. This research aimed to isolate and identify endophytic microorganisms in coal mining sites with potential to play a role in coal mining reclamation related to plant nutrient level. The method used in this research was exploration method, by determining sample points in former coal mining sites with high representation value. Samples were taken from 5 points in 3 different former mining sites located in East Penajam Paser Regency, East Borneo, with a total of 15 sample locations. Bacterial isolation was performed followed by characterization and identification using gram staining and calculation of comparable coefficients. To determine the percentage of comparable coefficients (Ss) of positive and negative similarities of characteristics from each bacterial species, the morphological and physiological test results of the Bacillus bacteria were used. The isolation results showed that there were 11 (eleven) isolates which could be identified into 11 types of bacteria which were potential as a nutrient level role based on the results of the characterization ex-coal mining sites; Bacillus pantothenticus, B. mycoides, B. firmus, B. brevis, B. stearothermophilus, B. anthracis, B. laterosporus, B. sphaericus, B. sphaericus, B. alvei, and B. firmus. All isolates came from Bacillus genus with similarity percentage at 65-70%.
- © 2021 The Authors. Published by Atlantis Press International B.V.
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Cite this article
TY - CONF AU - Yuni S. Rahayu AU - Yuliani AU - M. T. Asri PY - 2021 DA - 2021/12/16 TI - Isolation and Identification of Endophytic Bacteria Related to Plant Nutrient Level in Coal Mining Site from East Kalimantan Indonesia BT - Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021) PB - Atlantis Press SP - 485 EP - 491 SN - 2352-5401 UR - https://doi.org/10.2991/aer.k.211215.082 DO - 10.2991/aer.k.211215.082 ID - Rahayu2021 ER -