Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)

Isolation and Identification of Endophytic Bacteria Related to Plant Nutrient Level in Coal Mining Site from East Kalimantan Indonesia

Authors
Yuni S. Rahayu1, *, Yuliani2, M. T. Asri3
1,2,3Department of Biology, Universitas Negeri Surabaya
*Corresponding author. Email: yunirahayu@unesa.ac.id
Corresponding Author
Yuni S. Rahayu
Available Online 16 December 2021.
DOI
https://doi.org/10.2991/aer.k.211215.082How to use a DOI?
Keywords
Coal mining area; Identification of endophytic microorganisms; East Borneo
Abstract

East Kalimantan has a very wide area for coal mining. However, not all coal mining companies carry out land reclamation before coal mine was abandoned. This marginal land is very potential to be utilized as productive land, although efforts must be done to minimize the main limiting factor in coal mining site, namely low soil fertility by utilizing endophytic bacteria found in ex-coal mining sites. This research aimed to isolate and identify endophytic microorganisms in coal mining sites with potential to play a role in coal mining reclamation related to plant nutrient level. The method used in this research was exploration method, by determining sample points in former coal mining sites with high representation value. Samples were taken from 5 points in 3 different former mining sites located in East Penajam Paser Regency, East Borneo, with a total of 15 sample locations. Bacterial isolation was performed followed by characterization and identification using gram staining and calculation of comparable coefficients. To determine the percentage of comparable coefficients (Ss) of positive and negative similarities of characteristics from each bacterial species, the morphological and physiological test results of the Bacillus bacteria were used. The isolation results showed that there were 11 (eleven) isolates which could be identified into 11 types of bacteria which were potential as a nutrient level role based on the results of the characterization ex-coal mining sites; Bacillus pantothenticus, B. mycoides, B. firmus, B. brevis, B. stearothermophilus, B. anthracis, B. laterosporus, B. sphaericus, B. sphaericus, B. alvei, and B. firmus. All isolates came from Bacillus genus with similarity percentage at 65-70%.

Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)
Series
Advances in Engineering Research
Publication Date
16 December 2021
ISBN
978-94-6239-486-5
ISSN
2352-5401
DOI
https://doi.org/10.2991/aer.k.211215.082How to use a DOI?
Copyright
© 2021 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Yuni S. Rahayu
AU  - Yuliani
AU  - M. T. Asri
PY  - 2021
DA  - 2021/12/16
TI  - Isolation and Identification of Endophytic Bacteria Related to Plant Nutrient Level in Coal Mining Site from East Kalimantan Indonesia
BT  - Proceedings of the International Joint Conference on Science and Engineering 2021 (IJCSE 2021)
PB  - Atlantis Press
SP  - 485
EP  - 491
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.211215.082
DO  - https://doi.org/10.2991/aer.k.211215.082
ID  - Rahayu2021
ER  -