Detection of Verocytotoxygenic Escherichia coli (VTEC) from Brilliant Green Lactose Bilebroth (BGLB) by Polymerase Chain Reaction (PCR)

DOI

10.2991/isessah-19.2019.35How to use a DOI?

Keywords

VTEC, dairy farm, BGLB, PCR, primer vt1, and vt2

Abstract

Verocytotoxygenic Escherichia coli (VTEC) is pathogenic bacteria for human and associated with dairy milk and dairy farm environment. VTEC isolation were carried out by inoculating in BGLB as enrichment media and then specified in Eosin Methylene Blue (EMB) agar. This study aim to identify VTEC isolated from BGLB using PCR method. Six VTEC sample were used in this study, and those samples were collected from :milk bulktank (n=1), hand swab before milking (n=1), and hand swab after milking (n=1), swab of milk filter (n=1), feces (n=1), and water from dairy drinking places (n=1). All samples were inoculated in BGLB, and then bacterial DNA were extracted. PCR amplification using specific primer of vt1 and vt2targeting on…..gene and….gene, with expected band at 130 bp and 779 bp, respectively. Positive results were sequenced and then analyzed using BLAST. Our results showed two out of six were positive of VTEC

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yatri Drastini
AU  - Rini Widayanti
AU  - Winda Ayuningtyas
PY  - 2019/12
DA  - 2019/12
TI  - Detection of Verocytotoxygenic Escherichia coli (VTEC) from  Brilliant Green Lactose Bilebroth (BGLB) by Polymerase Chain Reaction (PCR)
BT  - Proceedings of the Conference of the International Society for Economics and Social Sciences of Animal Health - South East Asia 2019 (ISESSAH-SEA 2019)
PB  - Atlantis Press
SP  - 3
EP  - 5
SN  - 2468-5739
UR  - https://doi.org/10.2991/isessah-19.2019.35
DO  - 10.2991/isessah-19.2019.35
ID  - Drastini2019/12
ER  -