Enhanced Protection of Hemibagrus nemurus and Clarias sp. in against Aeromonas hydrophila using Chitosan Coated Freeze-Dried Vaccine

DOI

10.2991/978-94-6463-942-1_6How to use a DOI?

Keywords

Aeromonas hydrophila; Catfish; Chitosan Coated Freeze-Dried Vaccine Motile Aeromonads Septicemia (MAS)

Abstract

The intensification of fish farming in Indonesia is at high risk of disease outbreaks, especially Aeromonas hydrophila bacterial infection that causes motile aeromonads septicemia (MAS). Vaccines are an essential alternative to disease control compared to chemicals or antibiotics. This study aims to evaluate the effectiveness of chitosan-coated A. hydrophila freeze-dried vaccine in catfish (Hemibagrus nemurus) and walking catfish (Clarias sp.), through oral and immersion methods. The vaccine was prepared with a bacterial concentration of 10¹¹ CFU/mL and chitosan 1.71% or 2. 04 g, then administered to fish for 7 days. The challenge test was carried out two weeks after vaccination using the LD₅₀ dose. The observed parameters included survival rate (SR), relative percent survival (RPS), phagocytosis index, antibody titer, and leukocyte differential. The results showed that the oral method gave the best results: SR reached 88.89% (catfish) and 78.89% (walking catfish), and RPS 71.42% and 73.97%, respectively, while SR in the control was 61.11% (catfish) and 18.89% (walking catfish). These results indicate that the chitosan-coated freeze-dried vaccine effectively increases immune protection against A. hydrophila and is feasible for widespread application in freshwater aquaculture.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Hessy Novita
AU  - Desy Sugiani
AU  - E. F. Wadjdy
AU  - Angela M. Lusiastuti
PY  - 2025
DA  - 2025/12/29
TI  - Enhanced Protection of Hemibagrus nemurus and Clarias sp. in against Aeromonas hydrophila using Chitosan Coated Freeze-Dried Vaccine
BT  - Proceedings of the 3rd International Seminar on Fish and Fisheries Sciences (ISFFS 2025)
PB  - Atlantis Press
SP  - 52
EP  - 61
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-942-1_6
DO  - 10.2991/978-94-6463-942-1_6
ID  - Novita2025
ER  -