Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)

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Motility of Bali Sexed Sperm Following Equilibration and Cryopreservation in Different Concentrations of Ethylene Glycol

Authors
Nurul Muchlisah Basyarah1, La Ode Nafiu2, Takdir Saili2, *
1Student of Master Program of Animal Science Study Program, Postgraduate Program, Universitas Halu Oleo
2Post Graduate Program of Animal Science Study Program, Universitas Halu Oleo
*Corresponding author. Email: takdir69@uho.ac.id
Corresponding Author
Takdir Saili
Available Online 29 March 2022.
DOI
10.2991/absr.k.220309.039How to use a DOI?
Keywords
Bali bull; sexed sperm; equilibration; freezing; thawing
Abstract

Freezing of bovine sperm has a critical point at the time of equilibration and freezing. Therefore, the equilibration duration and concentration of cryoprotectants that can maintain sperm during freezing and storage become determining factors for the success of bovine sperm freezing. This study aims to evaluate the effect of the interaction between equilibration duration and the concentration of ethylene glycol as cryoprotectants on the sperm motility of Bali bull sexed sperm both after equilibration and after freezing (post thawing motility). The study was conducted in February-March 2021 at the UPTD of the Center for Animal Breeding and Feed, the Food Crops and Livestock Service Office of Southeast Sulawesi Province. The material used was Bali bull aged three years and weighed 410kg as a source of semen. Factorial Completely randomized design (3x3) was used in this experiment. The first factor was the equilibration duration, consisting of 3 hours, 4 hours, and 5 hours. In contrast, the second factor was the concentration of ethylene glycol, which consisted of 5, 6, and 7%. The evaluated variable was sperm motility both after equilibration and post-thawing motility. Data were collected and tabulated, then analyzed using variance analysis, while the differences between treatments were compared using Duncan’s Multiple Region Test using the IBM Statistics SPSS 24 program. The results showed that the average motility of sperm after equilibration and post-thawing motility were 66,57±3,02% and 47.78±4.84%, respectively. Based on variance analysis, it was concluded that there was no interaction effect between equilibration duration and concentration of ethylene glycol on sperm motility both after equilibration and post thawing motility of Bali bull sexed sperm.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series
Advances in Biological Sciences Research
Publication Date
29 March 2022
ISBN
10.2991/absr.k.220309.039
ISSN
2468-5747
DOI
10.2991/absr.k.220309.039How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Nurul Muchlisah Basyarah
AU  - La Ode Nafiu
AU  - Takdir Saili
PY  - 2022
DA  - 2022/03/29
TI  - Motility of Bali Sexed Sperm Following Equilibration and Cryopreservation in Different Concentrations of Ethylene Glycol
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 187
EP  - 190
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.039
DO  - 10.2991/absr.k.220309.039
ID  - Basyarah2022
ER  -