Absolute Nucleic Acid Quantification System and Analysis

DOI

10.2991/itms-15.2015.410How to use a DOI?

Keywords

Digital nucleic acid amplification; Microfluidic; Dynamic range; Measurement Uncertainty

Abstract

Digital nucleic acid amplification and determination has become an attractive technique by its capable of high sensitivity and precision. With the development of microfluidic technique, this method becomes much easier to perform, with less sample requirement and cross contamination. The dynamic range of measurement is critical for digital nucleic acid amplification, and has greatly increased by improving the number of reactors. Here, a microfluidic device for digital nucleic acid amplification has been demonstrated, and the measurement uncertainty has been discussed. The paper’s aim is to present an absolute nucleic acid quantification system which is fabricated by nano-fabrication, and the dynamic range as well as measurement uncertainty of the system is also presented in the paper.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - B.F. Song
AU  - Q. Song
AU  - W. Jin
AU  - Q.C. Tian
AU  - Y. Mu
PY  - 2015/11
DA  - 2015/11
TI  - Absolute Nucleic Acid Quantification System and Analysis
BT  - Proceedings of the 2015 International Conference on Industrial Technology and Management Science
PB  - Atlantis Press
SP  - 1687
EP  - 1689
SN  - 2352-538X
UR  - https://doi.org/10.2991/itms-15.2015.410
DO  - 10.2991/itms-15.2015.410
ID  - Song2015/11
ER  -