Diversity analysis of paenibacillus cellulase

DOI

10.2991/macmc-17.2018.73How to use a DOI?

Keywords

cellulase; induction; enzyme activity; gel electrophoresis

Abstract

Cellulose is one of the most abundant biomass resources on the earth. The degradation of cellulose into bio-available sugar is of great significance to the development of bio-energy and bio-based chemicals as well as the comprehensive utilization of biomass resources. A large part of the cellulose degradation method is the use of cellulase degradation. Cellulase includes exo- -glucanase, -glucosidase, endo- -glucanase and the like. In general, cellulase produced by microorganisms in nature belongs to an inducible enzyme, and different substrates may induce a variety of cellulase. In this paper, we focused on the laboratory-screened paenibacillussp.LLZ1, and explored the changes of cellulase activity when the strain was cultured under different carbon sources, revealing the cellulase diversity produced by different carbon sources. The experimental results showed that when using sorbose and cellobiose as carbon sources, cellulase showed the highest activity of enzyme activity and CMC-Naenzyme activity. SDS-PAGE analysis showed that LLZ1 cellulase possessed the natural diversity and could be induced by different substrates Produce different -glucanase enzymes.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Wenxi Yue
AU  - Ming Sui
PY  - 2018/01
DA  - 2018/01
TI  - Diversity analysis of paenibacillus cellulase
BT  - Proceedings of the 2017 4th International Conference on Machinery, Materials and Computer (MACMC 2017)
PB  - Atlantis Press
SP  - 384
EP  - 389
SN  - 2352-5401
UR  - https://doi.org/10.2991/macmc-17.2018.73
DO  - 10.2991/macmc-17.2018.73
ID  - Yue2018/01
ER  -