Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering

Cloning and Site-specific Mutagenesis of DS Enzyme Gene of Corynebacterium glutamicum

Authors
Hansong Yu, Shuang Qu, Yuhua Wang, Chunhong Piao, Junmei Liu, Yaohui Hu
Corresponding Author
Hansong Yu
Available Online April 2015.
DOI
10.2991/mebe-15.2015.26How to use a DOI?
Keywords
Fermentation Engineering; Corynebacterium glutamicum; key enzyme of amino acid
Abstract

In order to obtain the higher activity DS enzyme through genetic engineering technology. The point mutation method is used to get a higher activity recombinant DS enzyme from Corynebacterium glutamicum. At last the point 183 was mutated from L to Q in LG-322.The mutation type is a fundermental of construct a genetic engineering which has high activity DS enzyme.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
Series
Advances in Engineering Research
Publication Date
April 2015
ISBN
978-94-62520-57-8
ISSN
2352-5401
DOI
10.2991/mebe-15.2015.26How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Hansong Yu
AU  - Shuang Qu
AU  - Yuhua Wang
AU  - Chunhong Piao
AU  - Junmei Liu
AU  - Yaohui Hu
PY  - 2015/04
DA  - 2015/04
TI  - Cloning and Site-specific Mutagenesis of DS Enzyme Gene of Corynebacterium glutamicum
BT  - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
PB  - Atlantis Press
SP  - 106
EP  - 110
SN  - 2352-5401
UR  - https://doi.org/10.2991/mebe-15.2015.26
DO  - 10.2991/mebe-15.2015.26
ID  - Yu2015/04
ER  -