Novel aptamer probe for detecting biphenol A based on fluorescence resonance energy transfer
- https://doi.org/10.2991/meic-14.2014.157How to use a DOI?
- FRET; aptame;BPA; fluorescence; deteting
Fluorescence resonance energy transfer (FRET) is the radiation process which the donor molecules at the excited state transferred capacity to the acceptor molecules via remote dipole. Aptamers can specially recognize its target molecules. Based on these principles, a sensitive and selective uorescent sensor for bisphenol A (BPA) has been designed. The fluorescence intensity of the sensing system increased gradually with the increasing of BPA concentration. F has a linear relationship with the logarithm of BPA concentration in the range of 0.1 nM - 10 nM concentration with a correlation coefficient (R) of 0.9986 and the detection limit of 0.07 nM ( defined as S/N=3). The proposed fluorescent sensor has good selectivity for BPA assay even in the presence of higher concentration of interferents.
- © 2014, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xiaojing Li AU - Minshi Chen AU - Jinlan Dai AU - Lijun Lu AU - Xi Tang AU - Zhexiang Zou AU - Siyuan Zheng AU - Huajian Liu PY - 2014/11 DA - 2014/11 TI - Novel aptamer probe for detecting biphenol A based on fluorescence resonance energy transfer BT - Proceedings of the 2014 International Conference on Mechatronics, Electronic, Industrial and Control Engineering PB - Atlantis Press SP - 702 EP - 705 SN - 2352-5401 UR - https://doi.org/10.2991/meic-14.2014.157 DO - https://doi.org/10.2991/meic-14.2014.157 ID - Li2014/11 ER -