Novel aptamer probe for detecting biphenol A based on fluorescence resonance energy transfer

DOI

10.2991/meic-14.2014.157How to use a DOI?

Keywords

FRET; aptame;BPA; fluorescence; deteting

Abstract

Fluorescence resonance energy transfer (FRET) is the radiation process which the donor molecules at the excited state transferred capacity to the acceptor molecules via remote dipole. Aptamers can specially recognize its target molecules. Based on these principles, a sensitive and selective uorescent sensor for bisphenol A (BPA) has been designed. The fluorescence intensity of the sensing system increased gradually with the increasing of BPA concentration. F has a linear relationship with the logarithm of BPA concentration in the range of 0.1 nM - 10 nM concentration with a correlation coefficient (R) of 0.9986 and the detection limit of 0.07 nM ( defined as S/N=3). The proposed fluorescent sensor has good selectivity for BPA assay even in the presence of higher concentration of interferents.

Copyright

© 2014, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiaojing Li
AU  - Minshi Chen
AU  - Jinlan Dai
AU  - Lijun Lu
AU  - Xi Tang
AU  - Zhexiang Zou
AU  - Siyuan Zheng
AU  - Huajian Liu
PY  - 2014/11
DA  - 2014/11
TI  - Novel aptamer probe for detecting biphenol A based on fluorescence resonance energy transfer
BT  - Proceedings of the 2014 International Conference on Mechatronics, Electronic, Industrial and Control Engineering
PB  - Atlantis Press
SP  - 702
EP  - 705
SN  - 2352-5401
UR  - https://doi.org/10.2991/meic-14.2014.157
DO  - 10.2991/meic-14.2014.157
ID  - Li2014/11
ER  -