In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

DOI

10.2991/miseic-19.2019.3How to use a DOI?

Keywords

seeds, source of explant, E. scaber, growth medium, in-vitro

Abstract

Elephantopus scaber plant contains various secondary metabolites, such as flavonoids, phenolics, saponins, terpenes, triterpenoids, sesquiterpenes lactones, elephantopyns, and deoxyelephantopin, which can act as antimicrobial, antifungal, and bioinsecticides. Elephantopus scaber can be potentially grown by tissue culture method and isolated its secondary compounds. But, the presence of tricomes at stem, leaf midrib, or leaves of E. scaber hinder its in-vitro propagation, because of it can facilitate contamination agents, such as fungi and bacteria. To avoid contamination, seeds were used as source of explants. The aim of this study was to describe the use of seeds as an alternative explant source of E. scaber in various culture growth medium and to determine the right medium for E. scaber callus induction. This research used four growth mediums: 1) MSO medium (with no additional Plant Growth Factor), 2) MSC medium (MS medium with additional charcoal), 3) MS + 0.5 mg/L BAP and 1 mg/L 2.4 D, and 4) MS + 1.5 mg/L Kinetin and 1.5 mg/L 2.4 D. There were 20 bottles media for experiment with five seed explants put into each bottle of medium, thus total of 100 seeds were planted. The propagation steps were sterilization of mature seeds, preparation and sterilization of growth medium, and inoculation of seeds. The observation of callus induction was performed for four weeks. The parameters observed in the research was the formation of the callus induced from seeds of E. scaber. The results showed that explants in 5 bottles had grown, 7 seeds were planted as seedlings, while seeds in 15 bottles showed no signs of growth. All seeds planted in MSC medium (20 bottles) produced seedlings and explants. About 82 calli from 20 bottles were induced on MS + 0.5 mg/L BAP and 1 mg/L 2.4 D, while 17 bottles of MS + 1.5 mg/L Kinetin and 1.5 mg/L 2.4 D produced 48 calli and 3 bottles produced seedlings. The results showed that seed explants of E. scaber could reduce microbe contamination because there was no tricomes in seeds, as opposite on the leaves during callus induction. Seeds of E. scaber could be effectively used as an explant for callus induction through in-vitro propagation. The most optimal medium for callus induction of E. scaber seed was MS + 0.5 mg/L BAP and 1 mg/L 2.4 D. These findings support recommendation for callus induction using the seeds for plants with numerous tricomes on their leaves, such as Elephantopus scaber.

Copyright

© 2019, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yuliani
AU  - Fida Rachmadiarti
AU  - Sari Kusuma Dewi
AU  - Mahanani Tri Asri
PY  - 2019/12
DA  - 2019/12
TI  - In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media
BT  - Proceedings of the Mathematics, Informatics, Science, and Education International Conference (MISEIC 2019)
PB  - Atlantis Press
SP  - 209
EP  - 212
SN  - 2352-538X
UR  - https://doi.org/10.2991/miseic-19.2019.3
DO  - 10.2991/miseic-19.2019.3
ID  - 2019/12
ER  -