Design and numerical simulation of an immunoisolation perfusion co-culture bioreactor for expansion of NSCs
- DOI
- 10.2991/mmebc-16.2016.406How to use a DOI?
- Keywords
- Immnuoisolation; Perfusion; NSCs; Expansion; Co-culture
- Abstract
Considering the immnuoisolation from the neural stem cells (NSCs) and endothelial progenitor cells (EPCs), the requirements of the carrier, shear tolerance and other factors, this study designed an immunoisolation perfusion co-culture bioreactor system, by dynamic perfusion culture environment of its upper and lower bi-levels and the immnuoisolation of intermediate polycarbonate membrane, combining the measure of embedding NSCs and EPCs with calcium alginate/gelatin microbeads, integrating hypoxic environment effectively to achieve the expansion of NSCs. The results show that the microbeads are more evenly distributed under dynamic perfusion conditions, the velocity of medium is slightly larger near polycarbonate membrane and the culture chamber wall surface, and has more balanced distribution of other regions, static pressure and dynamic pressure of the culture chamber are generally evenly distributed. Studies showed that the expansion of NSCs under perfusion conditions, is able to meet the mass transfer and provide a stable environment to the neural stem cell expansion.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Kedong Song AU - Siyu Jiang AU - Jiaxin Tian AU - Zeren Jiao AU - Yiwei Wang AU - Jiaquan Zhao AU - Tianqing Liu PY - 2016/06 DA - 2016/06 TI - Design and numerical simulation of an immunoisolation perfusion co-culture bioreactor for expansion of NSCs BT - Proceedings of the 2016 6th International Conference on Machinery, Materials, Environment, Biotechnology and Computer PB - Atlantis Press SP - 2021 EP - 2026 SN - 2352-5401 UR - https://doi.org/10.2991/mmebc-16.2016.406 DO - 10.2991/mmebc-16.2016.406 ID - Song2016/06 ER -