Tailoring Ginger Exosome-Like Nanoparticles: Enhancing Isolation Efficiency via Precipitation-Centrifugation Synergy

DOI

10.2991/978-94-6239-717-0_26How to use a DOI?

Keywords

GELNs; PEG-6000; differential centrifugation; nanoparticle optimization; biopharmaceutical characterization

Abstract

Plant-derived extracellular vesicles, particularly Ginger Exosome-Like Nanoparticles (GELNs), are emerging as potential natural nanocarriers for the delivery of bioactive compounds. The need for specialized ultracentrifugation equipment, suboptimal yield, and persistent macromolecular contaminations still hinders the present procedure for the isolation and separation of exosomes. To address these limitations, the present study aimed to develop a hybrid protocol combining differential centrifugation with PEG-6000 precipitation, offering a cost-effective alternative to optimize particle purity, functionality, and bioactive integrity. The present study was designed to systematically evaluate the effects of variations in PEG-6000 concentrations and centrifugation durations on GELNs characteristics. A multi-stage isolation workflow was employed: initial low-speed centrifugation (2,000-10,000 g) to remove cellular debris, followed by overnight PEG-6000 incubation and a final clarifying spin (8,000 g). Nanoparticle characterization included particle size analyzer, LC-HRMS/MS, and electron microscopy. Results demonstrated that 12% PEG-6000 with variation B, yielding monodisperse nanoparticles (Z-average: 420.0 nm; PDI: 0.475) and high colloidal stability (intercept: 0.926). The LC-HRMS/MS results demonstrated the presence of 6-gingerol and shogaol as primary therapeutic agents, alongside structural lipids sphinganine and α-linolenate, which stabilize the lipid bilayer. Morphological analysis via electron microscopy confirmed spherical, membrane-intact vesicles. The optimized protocol reduced ultracentrifugation dependency by ±60%, significantly lowering production costs while preserving EV integrity. These findings position GELNs as a dual-functional system, combining the anti-inflammatory potential of gingerol and shogaol with structural stability conferred by sphinganine and α-linolenate. The study establishes a scalable framework for GELNs production, unlocking potential in targeted therapeutic delivery systems derived from plant-based nanomaterials.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Rika Sari Dewi
AU  - Melva Louisa
AU  - Ni Made Dwi Sandhiutami
AU  - Irandi Putra Pratomo
AU  - Kurnia Agustini
PY  - 2026
DA  - 2026/06/25
TI  - Tailoring Ginger Exosome-Like Nanoparticles: Enhancing Isolation Efficiency via Precipitation-Centrifugation Synergy
BT  - Proceedings of the 19th International Conference on Quality in Research (QiR 2025)
PB  - Atlantis Press
SP  - 355
EP  - 368
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6239-717-0_26
DO  - 10.2991/978-94-6239-717-0_26
ID  - Dewi2026
ER  -