Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)

Search and Isolation of Microorganisms from the Rangifer Tarandus Rumen Possessing Mycotoxin Biodegradation Potential and Cellulolytic Activity

Authors
Elena Yildirim, Larisa Ilina, Timur Dunyashev, Dmitiy Sobolev
Corresponding Author
Elena Yildirim
Available Online 29 January 2020.
DOI
https://doi.org/10.2991/assehr.k.200113.169How to use a DOI?
Keywords
rangifer tarandus, rumen, mycotoxin biodegradation, cellulolytic microorganisms
Abstract
Rumen content of the Rangifer tarandus is a promising source for the search for bacteria with high biological activity. Our objective was to screen isolates with high biological activity from the rumen content of reindeer in the Nenets and Yamalo-Nenets Autonomous Okrug of Russia. A total of 63 associations of microorganisms were investigated. Most of the studied associations showed the ability to decompose cellulose in the form of filter paper on the 3rd-6th day of incubation. 52 isolates demonstrated decomposition properties of carboxymethyl cellulose (according to data on the 3rd day of incubation). The results of the analysis of the cellulolytic activity of ten isolates that were selected as a result of screening according to the method of Henderson, Horvath and Block in the Churlis modification showed the level of cellulose decomposition from 4.0±0.34 to 62.0±4.1 is a promising source for the search for bacteria with high biological activity. Our objective was to screen isolates with high biological activity from the rumen content of reindeer in the Nenets and Yamalo-Nenets Autonomous Okrug of Russia. A total of 63 associations of microorganisms were investigated. Most of the studied associations showed the ability to decompose cellulose in the form of filter paper on the 3rd-6th day of incubation. 52 isolates demonstrated decomposition properties of carboxymethyl cellulose (according to data on the 3rd day of incubation). The results of the analysis of the cellulolytic activity of ten isolates that were selected as a result of screening according to the method of Henderson, Horvath and Block in the Churlis modification showed the level of cellulose decomposition from 4.0±0.34 to 62.0±4.1%. Four promising isolates selected at the next stage had the ability to biodegrade at least three mycotoxins. The highest level of biodegradation was observed for aflatoxins and reached 75.1±3.9 is a promising source for the search for bacteria with high biological activity. Our objective was to screen isolates with high biological activity from the rumen content of reindeer in the Nenets and Yamalo-Nenets Autonomous Okrug of Russia. A total of 63 associations of microorganisms were investigated. Most of the studied associations showed the ability to decompose cellulose in the form of filter paper on the 3rd-6th day of incubation. 52 isolates demonstrated decomposition properties of carboxymethyl cellulose (according to data on the 3rd day of incubation). The results of the analysis of the cellulolytic activity of ten isolates that were selected as a result of screening according to the method of Henderson, Horvath and Block in the Churlis modification showed the level of cellulose decomposition from 4.0±0.34 to 62.0±4.1%. Four promising isolates selected at the next stage had the ability to biodegrade at least three mycotoxins. The highest level of biodegradation was observed for aflatoxins and reached 75.1±3.9%. The obtained experiments led to the conclusion that the isolates of microorganisms isolated from the reindeer rumen are capable of synthesizing cellulases and enzymes that biodegrade mycotoxins, which probably allows them to gain a competitive advantage in the rumen of a deer that consumes diets with an abundance of indigestible fiber and secondary metabolites with toxic properties. These strains are of undoubted interest for livestock (including dairy cattle and reindeer husbandry) with the aim of using them as environmentally friendly and safe probiotic feed additives.
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Cite this article

TY  - CONF
AU  - Elena Yildirim
AU  - Larisa Ilina
AU  - Timur Dunyashev
AU  - Dmitiy Sobolev
PY  - 2020
DA  - 2020/01/29
TI  - Search and Isolation of Microorganisms from the Rangifer Tarandus Rumen Possessing Mycotoxin Biodegradation Potential and Cellulolytic Activity
BT  - Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)
PB  - Atlantis Press
SP  - 199
EP  - 204
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.200113.169
DO  - https://doi.org/10.2991/assehr.k.200113.169
ID  - Yildirim2020
ER  -