Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)

Carbonyl Derivatives of Proteins–as Markers of Free Radical Processes in Dairy Products

Authors
Valeriy Vysokogorskiy, Galina Kryshkina, Oksana Lazareva, Natalya Strelchik
Corresponding Author
Valeriy Vysokogorskiy
Available Online 29 January 2020.
DOI
https://doi.org/10.2991/assehr.k.200113.129How to use a DOI?
Keywords
antioxidant activity, oxidative modification of proteins, carbonyl derivatives, pasteurized milk, dihydroquercetin, iron-induced oxidative destruction
Abstract

Improving methods for assessing the biological properties of milk is a currently important task. The level of carbonyl derivatives of proteins during iron-induced oxidative degradation of pasteurized milk proteins was used as a marker of the intensity of free radical processes. During incubation of milk under aerobic conditions for 48 hours, a 1.6 - 2.0 times increase of the content of carbonyl derivatives of proteins was found at all studied wavelengths (from 356 to 535 nm). Maximal increase in the level of carbonyl derivatives was observed at absorption wavelengths corresponding to neutral ketone dinitrophenylhydrazones and, especially, to basic ketone dinitrophenylhydrazones (by 80-100Improving methods for assessing the biological properties of milk is a currently important task. The level of carbonyl derivatives of proteins during iron-induced oxidative degradation of pasteurized milk proteins was used as a marker of the intensity of free radical processes. During incubation of milk under aerobic conditions for 48 hours, a 1.6 - 2.0 times increase of the content of carbonyl derivatives of proteins was found at all studied wavelengths (from 356 to 535 nm). Maximal increase in the level of carbonyl derivatives was observed at absorption wavelengths corresponding to neutral ketone dinitrophenylhydrazones and, especially, to basic ketone dinitrophenylhydrazones (by 80-100%). The addition of dihydroquercetin to the incubation medium significantly inhibited the formation of carbonyl derivatives of proteins; there was only a slight increase in dinitrophenylhydrazones by 15–25Improving methods for assessing the biological properties of milk is a currently important task. The level of carbonyl derivatives of proteins during iron-induced oxidative degradation of pasteurized milk proteins was used as a marker of the intensity of free radical processes. During incubation of milk under aerobic conditions for 48 hours, a 1.6 - 2.0 times increase of the content of carbonyl derivatives of proteins was found at all studied wavelengths (from 356 to 535 nm). Maximal increase in the level of carbonyl derivatives was observed at absorption wavelengths corresponding to neutral ketone dinitrophenylhydrazones and, especially, to basic ketone dinitrophenylhydrazones (by 80-100%). The addition of dihydroquercetin to the incubation medium significantly inhibited the formation of carbonyl derivatives of proteins; there was only a slight increase in dinitrophenylhydrazones by 15–25% at 428, 430 and 434 nm. Significant inhibition of the formation of carbonyl derivatives of milk proteins in the presence of a standard antioxidant confirms the free-radical nature of the formation of carbonyl derivatives of pasteurized milk proteins and indicates the possibility of using the evaluation of the level of carbonyl derivatives of milk proteins during aerobic incubation to detect the level of antioxidant activity of dairy products.

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© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Cite this article

TY  - CONF
AU  - Valeriy Vysokogorskiy
AU  - Galina Kryshkina
AU  - Oksana Lazareva
AU  - Natalya Strelchik
PY  - 2020
DA  - 2020/01/29
TI  - Carbonyl Derivatives of Proteins–as Markers of Free Radical Processes in Dairy Products
BT  - Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)
PB  - Atlantis Press
SP  - 13
EP  - 16
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.200113.129
DO  - https://doi.org/10.2991/assehr.k.200113.129
ID  - Vysokogorskiy2020
ER  -