Analysis Method of Lactoferrin Based on Uncoated Capillary Electrophoresis
- 10.2991/efood.k.210720.001How to use a DOI?
- Capillary electrophoresis; lactoferrin; analytical method; auxiliary air pressure; surfactant
The analysis of lactoferrin separation will be an important application in prospect. To develop a new strategy for the detection of lactoferrin, this work adopted the method of uncoated Capillary Electrophoresis (CE), through optimizing the parameters of background electrolyte, pH, surfactant, buffers of the system solution. The optimum lactoferrin separation by CE is 50 mM phosphates buffer added 6M urea. In positive voltage, lactoferrin was separated by CE at pH 4.0. In reverse voltage, lactoferrin can also be separated at pH 10.0 with 50 mM ammonium ace. The auxiliary air pressure was got involved to decrease the absorption by silica capillary through a range of gradient intensity of air pressure. By comparison with 1.2, 1.5 and 1.8 psi, the pressure of 0.5 psi can significantly alleviate the absorption. The best detection conditions of lactoferrin were obtained, which are pH 4.0, 50 mM phosphates with 6M urea, 0.5 psi auxiliary air pressure with the limit of quantification at 0.04 mg/mL and the limit of detection at 0.01 mg/mL. Finally, lactoferrin can be quantitatively detected well and effectively, which the standard curve is y = 117807x + 3018.61374.
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- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - JOUR AU - Hui Chen AU - Zhenyu Wang AU - Fengjiao Fan AU - Pujie Shi AU - Xianbing Xu AU - Ming Du AU - Cong Wang PY - 2021 DA - 2021/07/26 TI - Analysis Method of Lactoferrin Based on Uncoated Capillary Electrophoresis JO - eFood SP - 147 EP - 153 VL - 2 IS - 3 SN - 2666-3066 UR - https://doi.org/10.2991/efood.k.210720.001 DO - 10.2991/efood.k.210720.001 ID - Chen2021 ER -