Journal of Epidemiology and Global Health

Volume 7, Issue 4, December 2017, Pages 263 - 267

Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran

Authors
Yaser Fasihia, Somayeh Kiaeia, Davood Kalantar-Neyestanakib, *, D.kalantar@kmu.ac.ir
aStudent Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
bDepartment of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
*Corresponding author.
Corresponding Author
Davood Kalantar-NeyestanakiD.kalantar@kmu.ac.ir
Received 12 November 2016, Revised 10 August 2017, Accepted 17 August 2017, Available Online 30 August 2017.
DOI
https://doi.org/10.1016/j.jegh.2017.08.004How to use a DOI?
Keywords
MRSA; VRSA; SCCmec; spa type
Abstract

Spread of methicillin-resistant Staphylococcus aureus (MRSA) isolates is a worldwide problem. Molecular typing is a useful tool to understand MRSA epidemiology. Herein, we determined vancomycin-resistant, SCCmec and spa types among MRSA isolates recovered from healthcare and community-acquired infections in Kerman, Iran. A total of 170 S. aureus isolates were collected from different patients who were admitted to affiliated hospitals of Kerman University of Medical science. MRSA and vancomycin-resistant S. aureus (VRSA) isolates were detected by phenotypic methods. Polymerase chain reaction (PCR) technique was used for detection of mecA, vanA and vanB genes. Staphylococcal cassette chromosomemec (SCCmec) and spa typing were used for molecular typing of among MRSA isolates. Overall, 53% of isolates were considered as MRSA. Two MRSA isolates were resistant to vancomycin and vanA was detected in only one of VRSA isolates. SCCmec type III belonged to spa types t030 and t459 which they were the dominant spa types among community-associated MRSA (CA-MRSA) and healthcare-acquired MRSA (HA-MRSA) isolates. Our findings showed that the SCCmec type I and III spread from hospital settings to community, although the SCCmec type IV spread from community to healthcare systems. We have also reported VRSA isolates from hospitalized patients, therefore, appropriate policies should be enforced in order to prevent the spread of antibiotic resistance isolates in hospitals settings.

Copyright
© 2017 Ministry of Health, Saudi Arabia. Published by Elsevier Ltd.
Open Access
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Journal
Journal of Epidemiology and Global Health
Volume-Issue
7 - 4
Pages
263 - 267
Publication Date
2017/08/30
ISSN (Online)
2210-6014
ISSN (Print)
2210-6006
DOI
https://doi.org/10.1016/j.jegh.2017.08.004How to use a DOI?
Copyright
© 2017 Ministry of Health, Saudi Arabia. Published by Elsevier Ltd.
Open Access
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Cite this article

TY  - JOUR
AU  - Yaser Fasihi
AU  - Somayeh Kiaei
AU  - Davood Kalantar-Neyestanaki
PY  - 2017
DA  - 2017/08/30
TI  - Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran
JO  - Journal of Epidemiology and Global Health
SP  - 263
EP  - 267
VL  - 7
IS  - 4
SN  - 2210-6014
UR  - https://doi.org/10.1016/j.jegh.2017.08.004
DO  - https://doi.org/10.1016/j.jegh.2017.08.004
ID  - Fasihi2017
ER  -