Determination of Plasma Protein Binding by Microdialysis Coupled with Online Capillary Electrophoresis Electrochemiluminescence

DOI

10.2991/aeece-15.2015.174How to use a DOI?

Keywords

Ultrasonic microdialysis; Electrochemiluminescence; Capillary electrophoresis; Plasma protein binding; Chlorpheniramine maleate

Abstract

An ultrasonic microdialysis coupled with online capillary electrophoresis electrochemiluminescence method has been established for the sensitive determination of plasma protein binding. The drug and blood plasma were mixed in different molar ratios and incubated at 37 °C in 10 mmol/L phosphate buffer (pH 7.5) in a water bath. The concentration of unbound chlorpheniramine maleate was determined by CE-ECL. The conditions affecting separation and detection were investigated. Under the optimized conditions: ECL detection at 1.2V, dialysis time 1.5h, protein binding rate of chlorpheniramine maleate with human plasma protein were determinated. The human plasma protein binding rate of chlorpheniramine maleate was 72.3%.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Qian Xiang
AU  - Xiaodong Yang
AU  - Ying Gao
PY  - 2015/09
DA  - 2015/09
TI  - Determination of Plasma Protein Binding by Microdialysis Coupled with Online Capillary Electrophoresis Electrochemiluminescence
BT  - Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering
PB  - Atlantis Press
SP  - 844
EP  - 847
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-15.2015.174
DO  - 10.2991/aeece-15.2015.174
ID  - Xiang2015/09
ER  -