Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

Genetic Relationship between Rubus Parvifolius and R. Coreanus (Rubus, Rosaceae) based on Simple Sequence Repeat Markers

Authors
Yan Wang, Wen He, Jing Zhang, Tao Chen, Qing Chen, Hao-Ru Tang, Lin Liu, Xiao-Rong Wang
Corresponding Author
Yan Wang
Available Online February 2018.
DOI
https://doi.org/10.2991/bst-17.2018.16How to use a DOI?
Keywords
Rubus parvifolius L., R. coreanus Miq., SSR, Genetic structure.
Abstract

Rubus parvifolius L. and R. coreanus Miq. are two morphologically distinct, endemic wild brambles and sympatric in China. Genetic diversity and genetic structure of the two species and their putative hybrids were investigated by using 10 SSR markers selected from 31 markers. A total of 98 alleles were amplified and 4 to 14 alleles per locus were obtained among 55 individuals. Genetic distance calculated from SSR data ranged from 0.7999 to 0.9347 and genetic identity from 0.0675 to 0.2233. Compared to the R. parvifolius (Ho = 0.4978) and R. coreanus (Ho = 0.5237), almost the same level of observed heterozygosity was observed in the putative hybrid populations (Ho = 0.5447). This was consistent with the low level of genetic differentiation (0.015 to 0.0309) within species and strong gene flow (Nm = 7.7254) among species. Rubus parvifolius, R. coreanus and their putative hybrids were categorized into three groups by structure analysis. The AMOVA analysis revealed low genetic differentiation among species (putative hybrids), with only 3.13% of total variability partitioned among them. Based on these results, impact of hybridization and introgression on genetic diversity of R. parvifolius and the relationship between R. parvifolius and R. coreanus were mainly discussed.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
Series
Advances in Biological Sciences Research
Publication Date
February 2018
ISBN
978-94-6252-472-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-17.2018.16How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Yan Wang
AU  - Wen He
AU  - Jing Zhang
AU  - Tao Chen
AU  - Qing Chen
AU  - Hao-Ru Tang
AU  - Lin Liu
AU  - Xiao-Rong Wang
PY  - 2018/02
DA  - 2018/02
TI  - Genetic Relationship between Rubus Parvifolius and R. Coreanus (Rubus, Rosaceae) based on Simple Sequence Repeat Markers
BT  - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
PB  - Atlantis Press
SP  - 101
EP  - 109
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-17.2018.16
DO  - https://doi.org/10.2991/bst-17.2018.16
ID  - Wang2018/02
ER  -