Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)

Authors
Xun-Ju Liu, Yan Wang, Hao-Ru Tang, Qing Chen
Corresponding Author
Xun-Ju Liu
Available Online February 2018.
DOI
https://doi.org/10.2991/bst-17.2018.19How to use a DOI?
Keywords
Blackberry, CER1, Cloning and expression, Light irradiation
Abstract

To understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene CER1 was cloned from blackberry. The open reading frame of this gene was 1878 bp, encoding 625 amino acid residues with a molecular weight of 72.3 kD and a theoretical isoelectric point of 8.59. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the gene expression increased with the maturation of blackberry. With the increase of the ratio of red or blue light, the expression of CER1 gene firstly decreased and then increased. Under the condition of red and blue light at a ratio of 2:8, the expression of CER1 was the lowest. The reason may be related to the different mechanisms of photoreceptor response to red and blue light.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
Series
Advances in Biological Sciences Research
Publication Date
February 2018
ISBN
978-94-6252-472-9
ISSN
2468-5747
DOI
https://doi.org/10.2991/bst-17.2018.19How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Xun-Ju Liu
AU  - Yan Wang
AU  - Hao-Ru Tang
AU  - Qing Chen
PY  - 2018/02
DA  - 2018/02
TI  - Cloning and Expression Analysis of CER1 in Blackberry (Rubus Spp.)
BT  - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
PB  - Atlantis Press
SP  - 128
EP  - 133
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-17.2018.19
DO  - https://doi.org/10.2991/bst-17.2018.19
ID  - Liu2018/02
ER  -