Characterization of LEAFY in Rubus Coreanus and Its Phylogenetic Application in Rubus Species

DOI

10.2991/bst-17.2018.47How to use a DOI?

Keywords

Rubus, LEAFY, Clone, Phylogenetic application.

Abstract

Rubus coreanus is endemically distributed in Southwest China and has great potential for Rubus breeding due to its nutritional values and insects and pests' resistance. LEAFY, as a single copy nuclear gene in diploid plants, plays an important role in the taxonomy and phylogeny, containing three exons and two introns. Here we designed new primers to amplify LEAFY gene fragment based on gene sequences from apple, strawberry, pear and peach. The new designed primers were demonstrated to be applicable for several other Rubus species in Rubus genus. The full length of LEAFY fragment in R. coreanus was 1127 bp, including 319 bp at the 3' end of the second exon, 729 bp of the second intron, and 39 bp from the 5' end of the third exon. Its GC content was 30% while AT content counted for 70%. We further reconstructed the phylogenetic tree using several LEAFY gene from Rosaceae species. These results could provide effective tool to study the phylogenetic relationships within the Rubus genus.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dan Jiang
AU  - Hao-Ru Tang
AU  - Yan Wang
AU  - Qing Chen
PY  - 2018/02
DA  - 2018/02
TI  - Characterization of LEAFY in Rubus Coreanus and Its Phylogenetic Application in Rubus Species
BT  - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
PB  - Atlantis Press
SP  - 301
EP  - 305
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-17.2018.47
DO  - 10.2991/bst-17.2018.47
ID  - Jiang2018/02
ER  -