The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)

Isolation and Identification of Ethyl Acetate Fractions in Flour Banana Fruit (Musa troglodytarum L.)

Authors
Dolih Gozali, Resmi Mustarichi, Sandra Megantara, Ami Tjitraresmi, Fikri D. Alminda
Corresponding Author
Dolih Gozali
Available Online 8 June 2020.
DOI
https://doi.org/10.2991/ahsr.k.200523.018How to use a DOI?
Keywords
Musa troglodytarum L, isolation, steroid
Abstract

The use of traditional medicine is recommended by WHO to maintain of peoples health, preventing and treatment of diseases. Indonesia as a country that has biodiversity of banana, one of them is a tongka langit banana. Musa troglodytarum L. better known as banana tongka langit is one of the banana plants used by the community around Tasikmalaya to overcome various diseases such as diabetes mellitus, immune enhancers and overcome anemia. Previous research stated that the administration of ethyl acetate fraction from tongka langit banana can reduce blood glucose levels in mice induced by alloxan. Based on that, further study is needed to isolate and identify the compounds in the ethyl acetate fraction of ethanol extract of banana fruit tongka Langit (Musa troglodytarum L.). Simplicia maserated using ethanol 70%, then fractionated with LLE method using n-hexane, ethyl acetate and water, ethyl acetate fraction was isolated by using classical column chromatography and preparative thin layer method, and ensuring the isolation purity using two-way thin layer chromatography. Compound identification methods include organoleptic, thin layer chromatography (TLC) with specific spotting appearance, infrared spectrophotometry and mass spectroscopy. From the study four isolates were produced, but the MT-II-2 isolates had more results and were possible to be identified. The results of the identification group compound showed that MT-II-2 isolates were thought to be steroid. Infrared spectrophotometry results showed that isolates had functional groups C-O (Aldehyde), C-O (Carboxylate / Alcohol), C-H (Alkana), C-H Bend, C = C, C = O (Aldehyde), C-H and O-H. and mass spectroscopy results that the isolate has a molecular weight of 507,1436 m / z with predictions of the molecular formulas C31H23O7, C38H19O2, C24H27O12.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
Series
Advances in Health Sciences Research
Publication Date
8 June 2020
ISBN
978-94-6252-976-2
ISSN
2468-5739
DOI
https://doi.org/10.2991/ahsr.k.200523.018How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Dolih Gozali
AU  - Resmi Mustarichi
AU  - Sandra Megantara
AU  - Ami Tjitraresmi
AU  - Fikri D. Alminda
PY  - 2020
DA  - 2020/06/08
TI  - Isolation and Identification of Ethyl Acetate Fractions in Flour Banana Fruit (Musa troglodytarum L.)
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 67
EP  - 69
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.018
DO  - https://doi.org/10.2991/ahsr.k.200523.018
ID  - Gozali2020
ER  -