Microbial Diversity Responded to Application of Nitrification Inhibitor in Greenhouse Cucumber Soil for Three Years

DOI

10.2991/edep-18.2018.57How to use a DOI?

Keywords

Cucumber; Rhizosphere microbiome; Nitrification inhibitor; Greenhouse; Microbial ecology

Abstract

[Purpose] The Nitrification inhibitor plays a significant role in mitigation of climate change, leaching of nitrate nitrogen and improvement of agricultural productivity and quality in greenhouses. Here we show a strong correlation between soil microbes and cucumber root systems according to our observation in greenhouse in north China by application of nitrification inhibitor to cucumber soil. [Methods] The effects of environmental factors and the function of dominant flora were further explored by deep pyrosequencing of 16S rRNA. [Results] It was shown that Proteobacteria, Actinobacteria, Chloroflexi, Germmatimonadates, Acidobacteria and Nitrospirae were the most abundant bacteria among all 783942 bacterial 16S rRNA genes clustering at the 97% similarity level. Furthermore, the Chao1,Shannon and Simpson index of AOA diversity, as well as the ACE and Chao1 index of AOB diversity with In-1 and In-2 treatment was not significantly different with treatments FP. Reducing the use of chemical fertilizers and organic fertilizers by 25% (OPT) significantly reduces the microbial diversity in the soil. In addition,it was found pH and Organic matter play dominant role in community structure among. [Conclusions] The application of nitrification inhibitors for three years did not cause negative effects on the microbial diversity in soil, and it still maintained the richness and evenness of the diversity in greenhouse soil. This work provides deeper understanding for evaluation of managing nitrogen conversion process and its effect on environment in greenhouse soil.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Ling WANG
AU  - Bo-Wen LI
AU  - Meng-Chao LIU
AU  - Guo-Yin ZHANG
AU  - Shi-You SUN
AU  - Ou-Ya ZHAO
PY  - 2018/10
DA  - 2018/10
TI  - Microbial Diversity Responded to Application of Nitrification Inhibitor in Greenhouse Cucumber Soil for Three Years
BT  - Proceedings of the 2018 International Conference on Energy Development and Environmental Protection (EDEP 2018)
PB  - Atlantis Press
SP  - 363
EP  - 371
SN  - 2352-5401
UR  - https://doi.org/10.2991/edep-18.2018.57
DO  - 10.2991/edep-18.2018.57
ID  - WANG2018/10
ER  -