Proceedings of the 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)

The Role of Myocardin in Platelet-derived Growth Factor-BB-induced Phenotypic Switching of Vascular Smooth Muscle Cells

Authors
Ying Yuan, Tiefeng Song, Huiqin Wang, Liwen Huang, Nan Wang
Corresponding Author
Ying Yuan
Available Online November 2016.
DOI
https://doi.org/10.2991/icache-16.2016.13How to use a DOI?
Keywords
Vascular smooth muscle cells - Proliferative phenotype - Myocardin - PDGF-BB
Abstract
In recent years, numerous studies confirm that platelet-derived growth factor-BB (PDGF-BB) is involved in the development of atherosclerosis and the regulation of human aorta VSMC (HA-VSMC) proliferation. Myocardin is an essential transcription factor that can activate the tran-scription of some smooth muscle marker genes. Our results showed that HA-VSMCs treated with PDGF-BB exhibited morphological changes from spindle-shaped to polygonal shape. The expres-sion of proliferation marker gene CyclinD1, osteopontin (OPN), proliferating cell nuclear antigen (PCNA) were increased in PDGF-BB-treated HA-VSMCs. 5-ethynyl-2'-deoxyuridine incorpo-ration assay (EdU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed that cell viability and proliferative ability were increased significantly in PDGF-BB treated HA-SMCs. Next, the expression of transcription factor myocardin was decreased in PDGF-BB-treated HA-VSMCs. These results preliminarily demonstrated the role of myocardin in phenotypic switching of PDGF-BB-induced HA-VSMCs.
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Proceedings
2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)
Part of series
Advances in Engineering Research
Publication Date
November 2016
ISBN
978-94-6252-255-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/icache-16.2016.13How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Ying Yuan
AU  - Tiefeng Song
AU  - Huiqin Wang
AU  - Liwen Huang
AU  - Nan Wang
PY  - 2016/11
DA  - 2016/11
TI  - The Role of Myocardin in Platelet-derived Growth Factor-BB-induced Phenotypic Switching of Vascular Smooth Muscle Cells
BT  - 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)
PB  - Atlantis Press
SP  - 70
EP  - 73
SN  - 2352-5401
UR  - https://doi.org/10.2991/icache-16.2016.13
DO  - https://doi.org/10.2991/icache-16.2016.13
ID  - Yuan2016/11
ER  -