The Role of Myocardin in Platelet-derived Growth Factor-BB-induced Phenotypic Switching of Vascular Smooth Muscle Cells
Ying Yuan, Tiefeng Song, Huiqin Wang, Liwen Huang, Nan Wang
Available Online November 2016.
- https://doi.org/10.2991/icache-16.2016.13How to use a DOI?
- Vascular smooth muscle cells - Proliferative phenotype - Myocardin - PDGF-BB
- In recent years, numerous studies confirm that platelet-derived growth factor-BB (PDGF-BB) is involved in the development of atherosclerosis and the regulation of human aorta VSMC (HA-VSMC) proliferation. Myocardin is an essential transcription factor that can activate the tran-scription of some smooth muscle marker genes. Our results showed that HA-VSMCs treated with PDGF-BB exhibited morphological changes from spindle-shaped to polygonal shape. The expres-sion of proliferation marker gene CyclinD1, osteopontin (OPN), proliferating cell nuclear antigen (PCNA) were increased in PDGF-BB-treated HA-VSMCs. 5-ethynyl-2'-deoxyuridine incorpo-ration assay (EdU) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed that cell viability and proliferative ability were increased significantly in PDGF-BB treated HA-SMCs. Next, the expression of transcription factor myocardin was decreased in PDGF-BB-treated HA-VSMCs. These results preliminarily demonstrated the role of myocardin in phenotypic switching of PDGF-BB-induced HA-VSMCs.
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- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ying Yuan AU - Tiefeng Song AU - Huiqin Wang AU - Liwen Huang AU - Nan Wang PY - 2016/11 DA - 2016/11 TI - The Role of Myocardin in Platelet-derived Growth Factor-BB-induced Phenotypic Switching of Vascular Smooth Muscle Cells BT - 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016) PB - Atlantis Press SP - 70 EP - 73 SN - 2352-5401 UR - https://doi.org/10.2991/icache-16.2016.13 DO - https://doi.org/10.2991/icache-16.2016.13 ID - Yuan2016/11 ER -