LncRNA MEG3 Is Involved in Endothelial Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

DOI

10.2991/icache-16.2016.14How to use a DOI?

Keywords

long non-coding RNA, MEG3, Mesenchymal stem cells, Endothelial cells, Differentiation

Abstract

The role of long non-coding RNA in endothelial differentiation of MSCs remains poorly understood. In this study, the levels of several lncRNAs including MEG3, MALAT1, SNHG5, lnc00657 and TUG1, which are high expressed in endothelial cells, were detected in hMSCs and HUVECs by real-time PCR. The results showed that lncRNA MEG3 was significantly high expressed in HUVECs, compared with hMSCs. hMSCs treated with VEGF and bFGF can exhibit the increase of endothelial cell marker CD31 level and also the increase of MEG3, suggesting that MEG3 could be involved in the endothelial differentiation of hMSCs. Finally, blockdown of MEG3 using siRNA of MEG3 led to the decrease of CD31 expression and the inhibition of hMSCs differentiation into endothelial cells.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Na Huang
AU  - Rui Zhang
AU  - Danyang Shi
AU  - Doudou Li
AU  - Hongpeng He
AU  - Hao Zhou
AU  - Tong-Cun Zhang
AU  - Nan Wang
PY  - 2016/11
DA  - 2016/11
TI  - LncRNA MEG3 Is Involved in Endothelial Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
BT  - Proceedings of the 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)
PB  - Atlantis Press
SP  - 74
EP  - 77
SN  - 2352-5401
UR  - https://doi.org/10.2991/icache-16.2016.14
DO  - 10.2991/icache-16.2016.14
ID  - Huang2016/11
ER  -