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Proceedings of the 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)

LncRNA MEG3 Is Involved in Endothelial Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Authors
Na Huang, Rui Zhang, Danyang Shi, Doudou Li, Hongpeng He, Hao Zhou, Tong-Cun Zhang, Nan Wang
Corresponding Author
Na Huang
Available Online November 2016.
DOI
https://doi.org/10.2991/icache-16.2016.14How to use a DOI?
Keywords
long non-coding RNA, MEG3, Mesenchymal stem cells, Endothelial cells, Differentiation
Abstract
The role of long non-coding RNA in endothelial differentiation of MSCs remains poorly understood. In this study, the levels of several lncRNAs including MEG3, MALAT1, SNHG5, lnc00657 and TUG1, which are high expressed in endothelial cells, were detected in hMSCs and HUVECs by real-time PCR. The results showed that lncRNA MEG3 was significantly high expressed in HUVECs, compared with hMSCs. hMSCs treated with VEGF and bFGF can exhibit the increase of endothelial cell marker CD31 level and also the increase of MEG3, suggesting that MEG3 could be involved in the endothelial differentiation of hMSCs. Finally, blockdown of MEG3 using siRNA of MEG3 led to the decrease of CD31 expression and the inhibition of hMSCs differentiation into endothelial cells.
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Proceedings
2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)
Part of series
Advances in Engineering Research
Publication Date
November 2016
ISBN
978-94-6252-255-8
ISSN
2352-5401
DOI
https://doi.org/10.2991/icache-16.2016.14How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Na Huang
AU  - Rui Zhang
AU  - Danyang Shi
AU  - Doudou Li
AU  - Hongpeng He
AU  - Hao Zhou
AU  - Tong-Cun Zhang
AU  - Nan Wang
PY  - 2016/11
DA  - 2016/11
TI  - LncRNA MEG3 Is Involved in Endothelial Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
BT  - 2016 2nd International Conference on Architectural, Civil and Hydraulics Engineering (ICACHE 2016)
PB  - Atlantis Press
SP  - 74
EP  - 77
SN  - 2352-5401
UR  - https://doi.org/10.2991/icache-16.2016.14
DO  - https://doi.org/10.2991/icache-16.2016.14
ID  - Huang2016/11
ER  -