Coating PAMAM dendrimer onto PDMS microfluidic chip to resist nonspecific Biomolecules adsorption

DOI

10.2991/icaemt-15.2015.41How to use a DOI?

Keywords

PAMAM dendrimer; microfluidic chip; caoting; hydrophilicity; adsorption

Abstract

The surface of poly(dimethylsiloxane)(PDMS) microfluidic chips were modified using polyamidoamine (PAMAM) dendrimer via physical coating. The results showed that the surface of the modified chips was coated by a dense, uniform, continuous, hydrophilicity layer of PAMAM. The hydrophilic of the chip surface was markedly improved. The contact angle of the chips modified decreased from108.9° to 46.1°. Adenosine and L-lysine were separated via the modified PDMS microfluidic chips. Compared with unmodified chips, the modified chips successfully separated the two biomolecules. The separation efficiencies of adenosine and L-lysine were 7.19×104 Plates/m and 8.111×104 Plates/m respectively, and the resolutions(RS) was 4.03. This research may provide a new and effective method to resist nonspecific adsorptionon the PDMS surface and the application of PDMS microfluidic chips in the determination of trace biomolecules.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dong Lin
AU  - Qin Zhao
AU  - Miaomiao Yan
AU  - Giuge Hou
PY  - 2015/08
DA  - 2015/08
TI  - Coating PAMAM dendrimer onto PDMS microfluidic chip to resist nonspecific Biomolecules adsorption
BT  - Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology
PB  - Atlantis Press
SP  - 204
EP  - 208
SN  - 2352-5401
UR  - https://doi.org/10.2991/icaemt-15.2015.41
DO  - 10.2991/icaemt-15.2015.41
ID  - Lin2015/08
ER  -