A Powerful Approach of Live Digital Data Communication in Biomedical Research and Teaching:Detection of Various Impaired Vascular Perfusion in Zebrafish Knockdown Models by Video Imaging
Ping-Pin Zheng, Rob Willemsen, Johan M Kros
Available Online August 2013.
- https://doi.org/10.2991/icaicte.2013.173How to use a DOI?
- video imaging, blood circulation, zebrafish
- There are various zebrafish models with cardiovascular defects which adequately mimic the impaired circulation and tissue perfusion of various human cardiovascular diseases. Zebrafish embryos/larvae are optically transparent, and the systemic blood circulation can be recorded by using a microscope with video imaging. We detected a series of circulatory defects in our caldesmon and glucose transport 1 knockdown zebrafish models, including arteriovenous (AV) shunting, collateral circulation, AV fusion, vessel bifurcation, reduced or depleted regional perfusion, sinus venous (SV) rupture, and more. The quick detection by simple video imaging of various pathological states of the blood circulation in the living zebrafish embryos/larvae is non-invasive and cost-effective. The method is suitable for large scale screening of altered blood circulation in various zebrafish models with impaired cardiovascular development. This is a powerful approach of live digital data communication in biomedical research and teaching.
- Open Access
- This is an open access article distributed under the CC BY-NC license.
Cite this article
TY - CONF AU - Ping-Pin Zheng AU - Rob Willemsen AU - Johan M Kros PY - 2013/08 DA - 2013/08 TI - A Powerful Approach of Live Digital Data Communication in Biomedical Research and Teaching:Detection of Various Impaired Vascular Perfusion in Zebrafish Knockdown Models by Video Imaging BT - 2013 International Conference on Advanced ICT and Education (ICAICTE-13) PB - Atlantis Press SP - 853 EP - 855 SN - 1951-6851 UR - https://doi.org/10.2991/icaicte.2013.173 DO - https://doi.org/10.2991/icaicte.2013.173 ID - Zheng2013/08 ER -