Emotional regulation research is an important front hotspot of the emotional studies in recent years as well as the occupation self-efficacy of teachers. This research is a combination of these two aspects, which is from the different classification of emotion regulation method to the standard of self-efficiency of the college teacher. Through the experiment, it will be systematically investigated the effect of emotional experience to college teachers from the high self-efficiency, low self-efficiency and diverse emotion regulation methods. The research result indicates that firstly, there will be different effect of emotional subjective feelings if it has various emotion regulation methods. Evaluation of neglect will effectively weaken the subjective feelings of negative emotionality. Besides, evolution of value will enhance the subjective feelings of negative emotionality. Secondly, the difference of occupation self-efficiency level will lead a disparate impact of negative emotion subjective perception and high efficiency has little influence to negative emotion. It is efficiency to induce certainly intensive negative emotions according to laboratory technology of emotions evoking. 4. It has no relationship with teacher professional self-efficacy levels and gender. 5. The interactive effect between self-efficacy level and emotion regulation style is not obvious.