Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)

Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect

Authors
Litianyi Tang, Hong Liu
Corresponding Author
Litianyi Tang
Available Online May 2018.
DOI
10.2991/icammce-18.2018.1How to use a DOI?
Keywords
Analytical Chemistry; Photonic crystal, Fluorescence enhancement, Coffee-ring, Nano-imprint, POCT
Abstract

In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keeps growing with the increase of complexes concentration.As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)
Series
Advances in Engineering Research
Publication Date
May 2018
ISBN
10.2991/icammce-18.2018.1
ISSN
2352-5401
DOI
10.2991/icammce-18.2018.1How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Litianyi Tang
AU  - Hong Liu
PY  - 2018/05
DA  - 2018/05
TI  - Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect
BT  - Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)
PB  - Atlantis Press
SP  - 1
EP  - 5
SN  - 2352-5401
UR  - https://doi.org/10.2991/icammce-18.2018.1
DO  - 10.2991/icammce-18.2018.1
ID  - Tang2018/05
ER  -