Proceedings of the 2019 International Conference on Electronical, Mechanical and Materials Engineering (ICE2ME 2019)

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New Collagen Scaffolds with Extra Cellular Matrix Derived from Treatments of Supercritical Fluid and Protease in Low Temperature

Authors
Ciao-Yi Syu, Yu-Lin Shen, Cong-Han Xiao, Tzu-Pei Lee, Meng-Jen Yang, Ing-Lin Chang, Ching-Cheng Huang
Corresponding Author
Ching-Cheng Huang
Available Online March 2019.
DOI
10.2991/ice2me-19.2019.44How to use a DOI?
Keywords
ECM; enzymes; SCF
Abstract

Nowadays, tissue-engineering was important and popular which combine medical applications and engineering materials knowledge, just like cell extraction process was employed to remove the cellular components from porcine dermal, leaving a framework of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans (GAGs). A cell extraction process was used to remove cellular components by porcine dermal. Varying pore sizes and porosities of the acellular tissues were then created using enzymes and collagenase. The porcine dermal must remove fat and other impurities almost by supercritical fluid(SCF), otherwise adipose tissue will become rot immediately. For collagen scaffold samples, dermis were hydrolyzed by enzymes of different concentration, in this way it can gain state completely and clearly. Electron microscopy revealed effect in ECM of porcine dermal tissue via supercritical fluid and different concentrations enzymes. This suggests the enzymes concentration and when you put porcine dermal in SCF was mightily important for effect of the collagen.

Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2019 International Conference on Electronical, Mechanical and Materials Engineering (ICE2ME 2019)
Series
Advances in Engineering Research
Publication Date
March 2019
ISBN
10.2991/ice2me-19.2019.44
ISSN
2352-5401
DOI
10.2991/ice2me-19.2019.44How to use a DOI?
Copyright
© 2019, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Ciao-Yi Syu
AU  - Yu-Lin Shen
AU  - Cong-Han Xiao
AU  - Tzu-Pei Lee
AU  - Meng-Jen Yang
AU  - Ing-Lin Chang
AU  - Ching-Cheng Huang
PY  - 2019/03
DA  - 2019/03
TI  - New Collagen Scaffolds with Extra Cellular Matrix Derived from Treatments of Supercritical Fluid and Protease in Low Temperature
BT  - Proceedings of the 2019 International Conference on Electronical, Mechanical and Materials Engineering (ICE2ME 2019)
PB  - Atlantis Press
SP  - 196
EP  - 199
SN  - 2352-5401
UR  - https://doi.org/10.2991/ice2me-19.2019.44
DO  - 10.2991/ice2me-19.2019.44
ID  - Syu2019/03
ER  -