Determination of sulfonamide antibiotics in sludge by ultrasonic solid phase extraction and HPLC
- DOI
- 10.2991/iceesd-17.2017.118How to use a DOI?
- Keywords
- Ultrasonic solid phase extraction -HPLC; Sulfonamide antibiotics; High performance liquid chromatography (HPLC); Sludge
- Abstract
In order to improve the recovery rate of sulfonamide antibiotics in sludge, and to optimize the extraction and determination methods of sulfonamides antibiotics in sludge, ultrasonic solid phase extraction -HPLC was used for the detection of sulfonamides antibiotics in sludge. First the determination of the test factors by single factor test was conducted, then the extraction conditions were optimized by orthogonal experiment. When the extraction ratio of V (acetonitrile): V (water) was =1: 3, oscillation time was 20 min, ultrasonic frequency was 35 Hz, ultrasonic time was 15 min, and extraction agent was 20 ml, when the average content of extraction of sulfonamide antibiotics reached maximum, the recovery rates of Sulfadiazine, and sulfamethazine antibiotics were: 75.50% and 72.79% respectively. This indicates that the detection method can determine the content of sulfonamide antibiotics in the sludge effectively.
- Copyright
- © 2017, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xiaoxia Cao AU - Dengxin Li PY - 2017/04 DA - 2017/04 TI - Determination of sulfonamide antibiotics in sludge by ultrasonic solid phase extraction and HPLC BT - Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017) PB - Atlantis Press SP - 652 EP - 658 SN - 2352-5401 UR - https://doi.org/10.2991/iceesd-17.2017.118 DO - 10.2991/iceesd-17.2017.118 ID - Cao2017/04 ER -