Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)

Purification and characterization of Kunitz trypsin inhibitor from soybean

Authors
Chunmei Gu, Shujun Li, Xinxiu Song
Corresponding Author
Chunmei Gu
Available Online April 2017.
DOI
https://doi.org/10.2991/iceesd-17.2017.119How to use a DOI?
Keywords
soybean; Kunitz trypisin inhibitor; purification; characterization
Abstract
In this paper, a Kunitz trypsin inhibitor from soybean meal was isolated to apparent homogeneity by a combination of phosphoric acid extraction, heat treatment, ammonium sulfate precipitation, ion exchange chromatography, affinity chromatography and gel filtration. The results showed that the specific activity of 4733 Uúmg-1 and purification fold of 72.39 were obtained. The purified Kunitz trypsin inhibitor appeared a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined as 22907.51Da by MALDI-TOF. Partial amino acid sequence of the purified protein from Edman degration showed a high degree of homology with various members of the Kunitz-inhibitor family.
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Proceedings
2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)
Part of series
Advances in Engineering Research
Publication Date
April 2017
ISBN
978-94-6252-328-9
ISSN
2352-5401
DOI
https://doi.org/10.2991/iceesd-17.2017.119How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - CONF
AU  - Chunmei Gu
AU  - Shujun Li
AU  - Xinxiu Song
PY  - 2017/04
DA  - 2017/04
TI  - Purification and characterization of Kunitz trypsin inhibitor from soybean
BT  - 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)
PB  - Atlantis Press
SP  - 659
EP  - 666
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceesd-17.2017.119
DO  - https://doi.org/10.2991/iceesd-17.2017.119
ID  - Gu2017/04
ER  -