Effect of pH on Clotting Properties from Moringa Oleifera Seeds with Focused Microwave-Assisted Soxhlet Extraction Method as Milk Coagulant

DOI

10.2991/978-94-6463-116-6_49How to use a DOI?

Keywords

Moringa oleifera seeds; FMASE; pH; milk casein coagulant

Abstract

This study aimed to determine the optimal pH for Moringa oleifera seeds extract as a milk casein coagulant in terms of milk-clotting activity (MCA), caseinolytic activity (CA), ratio MCA/CA, and coagulation time. Moringa oleifera seeds extraction was obtained using the Focused Microwave-Assisted Soxhlet Extraction (FMASE) method. Milk-clotting activity was analyzed using skim milk. Caseinolytic activity was assessed using sodium caseinate as substrates. The effects of pH (5–8) on Moringa oleifera seeds enzyme activities were determined using experimental design of Completely Randomized Design (CRD) method with 5 treatments, maceration extract (P0), FMASE extract at pH 5 (T₁), 6 (T₂), 7 (T₃) and 8 (T₄) with 3 times replications. The significant influence will be tested using Duncan test. The results showed that the difference in pH atmosphere had a very significant effect (P < 0.01) on MCA, CA, MCI, and coagulation time. The pH 5 setting on the addition of Moringa oleifera seeds extract as a milk coagulant showed the optimum results at MCA 69.68 U/ml and MCI 3209.09, low yields at CA 0.02. U/ml, and gives a short time in the milk coagulation process of 14.35 s. The conclusion of this study is that adjusting the pH atmosphere in an increasingly acidic atmosphere will increase the work of the protease enzyme in Moringa oleifera seeds extract. pH 5 and 6 Moringa oleifera seeds extract can work optimally and will decrease at pH 7.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Aurelia Aprilianty
AU  - Rizqi Nuraini
AU  - Novia Pebriani
AU  - Gita Apriani
AU  - Abdul Manab
AU  - Khotibul Umam Awwaly
AU  - Mustakim
AU  - Ria Dewi Andriani
PY  - 2023
DA  - 2023/03/08
TI  - Effect of pH on Clotting Properties from Moringa Oleifera Seeds with Focused Microwave-Assisted Soxhlet Extraction Method as Milk Coagulant
BT  - Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
PB  - Atlantis Press
SP  - 391
EP  - 404
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-116-6_49
DO  - 10.2991/978-94-6463-116-6_49
ID  - Aprilianty2023
ER  -