Antiaging Potency of Centella Asiatica Extract on Fibroblast Cells of Rattus Norvegicus Fetus by in Vitro and in Silico Approach

Zuliati Ningsih; Bayyinatul Muchtaromah; Maharani Retna Duhita; Robiatul Adawiyah; Farah Fauzia Maulahibati; Alfiah Hayati; Tanjina Sharmin

doi:10.2991/978-94-6463-148-7_45

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Antiaging Potency of Centella Asiatica Extract on Fibroblast Cells of Rattus Norvegicus Fetus by in Vitro and in Silico Approach

Authors

Zuliati Ningsih¹, Bayyinatul Muchtaromah¹^{, *}, Maharani Retna Duhita¹, Robiatul Adawiyah¹, Farah Fauzia Maulahibati¹, Alfiah Hayati², Tanjina Sharmin³

¹Master Program of Biology, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Malang, Indonesia

²Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia

³Faculty of Chemical Engineering, Fukuoka University, Fukuoka, Japan

^*Corresponding author. Email: bayyinatul@bio.uin-malang.ac.id

Corresponding Author

Bayyinatul Muchtaromah

Available Online 29 May 2023.

DOI: 10.2991/978-94-6463-148-7_45 How to use a DOI?
Keywords: Extract Centella asiatica (EkCa); Rat Fetal Fibroblast Cells; Confluency; Viability; Screening
Abstract: Aging is a physiological process that cannot be avoided, but can be prevented by giving antioxidants. Antioxidants can be obtained from Centella asiatica extract (EkCa). EkCa has secondary metabolites that have biological effects related to inflammation and prevent aging. The purpose of this study was to determine the anti-aging potential of EkCa in fibroblast cells of Rattus norvegicus fetus and which compounds that act as anti-aging on EkCa using in vitro and in silico approach. The method used in this study was completely randomized design with 5 treatments (control, 10%, 15%, 20%, 25%), each repeated 4 times on fibroblast cells of Rattus norvegicus fetus that were 18 days pregnant. In vitro approach used to determine the confluency and viability on fibroblast cells of Rattus norvegicus while in silico approach used to screening which active compounds that have the potential as anti-aging using Way2Drug PASS online software. Data analysis used One Way ANOVA followed by Duncan's test. The results showed that the highest level of confluency and viability was found in the 25% treatment (P < 0.05) and quercetin-3- arabinoside was the compound in EkCa that has the potential as anti-aging with Pa: 0.922 and Pi: 0.003. From the result, it can be conclude that EkCa has the potential as anti-aging on fibroblast cells of Rattus norvegicus.
Copyright: © 2023 The Author(s)
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
Series: Advances in Engineering Research
Publication Date: 29 May 2023
ISBN: 10.2991/978-94-6463-148-7_45
ISSN: 2352-5401
DOI: 10.2991/978-94-6463-148-7_45 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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ris enw bib

TY  - CONF
AU  - Zuliati Ningsih
AU  - Bayyinatul Muchtaromah
AU  - Maharani Retna Duhita
AU  - Robiatul Adawiyah
AU  - Farah Fauzia Maulahibati
AU  - Alfiah Hayati
AU  - Tanjina Sharmin
PY  - 2023
DA  - 2023/05/29
TI  - Antiaging Potency of Centella Asiatica Extract on Fibroblast Cells of Rattus Norvegicus Fetus by in Vitro and in Silico Approach
BT  - Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
PB  - Atlantis Press
SP  - 452
EP  - 459
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-148-7_45
DO  - 10.2991/978-94-6463-148-7_45
ID  - Ningsih2023
ER  -

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