Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

Food Contaminants in Tea Products from Kratom Leaves

Authors
Reza Y. Purwoko1, *, Syahrul Tuba2, Sri Idaiani1, Harimat Hendarwan1, Islamudin Ahmad3, Harryadin Mahardika4, Kevin Tandarto5, Caroline Oktarina6, Reganedgary Jonlean7
1Research Center for Pre-clinical and Clinical Medicine, The National Research and Innovation Agency (BRIN), Jakarta, Indonesia
2Department of Pharmacy, Faculty of Pharmacy, Indonesian Defense University, Jakarta, Indonesia
3Department of Pharmaceutical Sciences, Mulawarman University, Samarinda, Indonesia
4Military Medical Faculty, Indonesia Defense University, Tajur, Indonesia
5Department of Internal Medicine, Atma Jaya Catholic University, Jakarta, Indonesia
6Department of Dermatology and Venereology, University of Indonesia, Jakarta, Indonesia
7Tzu Chi Hospital Pantai Indah Kapuk, Jakarta, Indonesia
*Corresponding author. Email: drrezayp@yahoo.com
Corresponding Author
Reza Y. Purwoko
Available Online 1 March 2023.
DOI
10.2991/978-94-6463-112-8_23How to use a DOI?
Keywords
food contaminant; red kratom; white kratom; green kratom
Abstract

One of the plants that has been used for generations in Borneo is kratom or Mitragyna speciosa. The leaves are used as analgesics and antidepressants. The plant is grown for export to countries other than Indonesia where consumption of this plant as a beverage is still legal, generally in tea from leaves brewed with hot water or tea bags. Some researches found that tea products from kratom leaves contained potentially dangerous levels of toxic metals and microbes. This study aims to examine the food contaminants in tea products from three types of kratom tea leaves used by the people of Borneo: Red, white, and green kratom variants.Bacterial colony tests for red kratom samples produced 2.9 x 10–3 colony forming unit (CFU)/gram. In comparison, white kratom samples produced 9.9 x 10–3 CFU/gram and green kratom samples produced 2.9 x 10–3 CFU/ gram. White kratom samples produced the highest CFU compared to red and green samples. Red kratom samples produced an uncountable number of yeasts at 10–2 and 10–3 dilution, while at 10–4 dilution, it produced a total of 4.8 x 10–5 CFU/gram. White kratom samples produced 3.04 x 10–4 CFU/gram and green kratom samples produced 1.7 x 10–4 CFU/gram. Red kratom samples produced the highest number among the three samples, while green kratom produced the lowest number. Identification with other specific media, namely Eosin Methylene Blue Agar (EMBA) and Salmonella-Shigella Agar (SSA), produced negative results for all samples. The red samples produced the highest of 6.6% b/b sample compared to 6.1% of white and 5.2% of green samples. All samples produced a positive qualitative test of mitragynine alkaloid. White kratom samples showed the highest Cd and Cu contamination, green kratom samples showed the highest Pb contamination and red samples showed the highest Hg contamination. Before preclinical and human clinical trial, it is advised to sterilize herbal simplicias of kratom as they tend to induce bacterial and fungal colonization. In some countries where kratom beverages are still legal, before the leaves were prepared for sale as tea it is better to make a ready-to-drink hygiene product and develop a set of “Good Manufacturing Pratices (GMP)” for the kratom industry before exporting it abroad to avoid microbes and heavy metal contaminations.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
Series
Advances in Health Sciences Research
Publication Date
1 March 2023
ISBN
10.2991/978-94-6463-112-8_23
ISSN
2468-5739
DOI
10.2991/978-94-6463-112-8_23How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Reza Y. Purwoko
AU  - Syahrul Tuba
AU  - Sri Idaiani
AU  - Harimat Hendarwan
AU  - Islamudin Ahmad
AU  - Harryadin Mahardika
AU  - Kevin Tandarto
AU  - Caroline Oktarina
AU  - Reganedgary Jonlean
PY  - 2023
DA  - 2023/03/01
TI  - Food Contaminants in Tea Products from Kratom Leaves
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 231
EP  - 239
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_23
DO  - 10.2991/978-94-6463-112-8_23
ID  - Purwoko2023
ER  -