9-POHSA Reduces LPS-Stimulated TNF-α Expression in Rat Hepatocyte: The Involvement of NF-KB-p65 Subunit

Jiro H. Situmorang; Ching-Hui Loh; Chih -Yang Huang

doi:10.2991/978-94-6463-112-8_8

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9-POHSA Reduces LPS-Stimulated TNF-α Expression in Rat Hepatocyte: The Involvement of NF-KB-p65 Subunit

Authors

Jiro H. Situmorang¹^{, 2}^{, *}, Ching-Hui Loh³, Chih -Yang Huang¹^{, 4}^{, 5}^{, 6}^{, 7}

¹Cardiovascular and Mitochondrial Related Disease Research Center, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

²Center for Biomedical Research, National Research and Innovation Agency (BRIN), Cibinong, Indonesia

³Center for Aging and Health, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

⁴College of Medicine, China Medical University, Taichung, Taiwan

⁵Department of Medical Research, China Medical University Hospital, Taichung, Taiwan

⁶Department of Biotechnology, Asia University, Taichung, Taiwan

⁷Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan

^*Corresponding author. Email: jiro.hasegawa.situmorang@brin.go.id

Corresponding Author

Jiro H. Situmorang

Available Online 1 March 2023.

DOI: 10.2991/978-94-6463-112-8_8 How to use a DOI?
Keywords: FAHFA; Fatty liver; LPS; MAFLD; TNF-α; 9-POHSA
Abstract: Metabolic (dysfunction) associated fatty liver disease (MAFLD) is phenotypically displayed by a buildup of fat in the liver. Over time, a patient with MAFLD tends to develop the disease into worse states like liver fibrosis and cirrhosis. The transition from simple steatosis into steatohepatitis is marked by inflammation. Several reports have suggested that endotoxemia is one factor that activates hepatic inflammation in MAFLD. Elevated lipopolysaccharide (LPS) localization and tumor necrosis factor-α (TNF-α) upregulation are found in the liver of patients and animal models with MAFLD. Fatty acid ester of hydroxy fatty acids (FAHFAs), a new group of lipids, is beneficial for metabolic diseases such as diabetes. The most pronounced effects of FAHFAs are their antiinflammation activity due to their ability to prevent upregulation of proinflammatory cytokines upon infection. The present study was undertaken to examine the anti-inflammatory activity of palmitoleic acid ester of 9-hydroxystearic acid (9-POHSA), one of the major endogenous FAHFA species in rat hepatocytes stimulated by LPS. Clone9 rat hepatocytes were cultured in DMEM without serum for 12 h and treated with LPS for 24 h with various concentration ranging from 0.1 to 10 μg/mL. TNF-α, p65, and phospho-p65 expression was assessed by immunofluorescence and Western blot. With or without LPS, the Clone9 cells were pretreated with 9-POHSA for 1 h. LPS, in a concentration-dependent manner, increased TNF-α expression and time-dependently increased the nuclear translocation of p65. Pretreatment of 9-POHSA inhibited the p65 translocation into the nucleus, TNF-α, and phospho-p65 expression. The results suggest that 9-POHSA exerts its anti-inflammatory activity by inhibiting p65 NF-kB related pathway.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
Series: Advances in Health Sciences Research
Publication Date: 1 March 2023
ISBN: 10.2991/978-94-6463-112-8_8
ISSN: 2468-5739
DOI: 10.2991/978-94-6463-112-8_8 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Jiro H. Situmorang
AU  - Ching-Hui Loh
AU  - Chih -Yang Huang
PY  - 2023
DA  - 2023/03/01
TI  - 9-POHSA Reduces LPS-Stimulated TNF-α Expression in Rat Hepatocyte: The Involvement of NF-KB-p65 Subunit
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 75
EP  - 81
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_8
DO  - 10.2991/978-94-6463-112-8_8
ID  - Situmorang2023
ER  -

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