The Nitric Oxide-Induced Immune Effect After Temulawak (Curcuma Xanthorrhizae Roxb.) Starch Treatment on Cyclophosphamide-Induced Rats

DOI

10.2991/978-94-6463-112-8_66How to use a DOI?

Keywords

Immunostimulant activity; cyclophosphamide; temulawak (Curcuma xanthorrhizae RoxB.) starch; nitric oxide

Abstract

Temulawak (Curcuma xanthorrhizae RoxB.) is known as an immunostimulant medicinal herb, however its starch has not been extensively investigated. Therefore, this study aimed to assess the immunostimulant effects of temulawak starch (TS) in cyclophosphamide (CYD)-induced Sprague-Dawley male rats. Temulawak starch (TS) were prepared with simple method using alpha-amylase enzyme to obtain the polysaccharide fraction. The quality of TS were evaluated using dextrose equivalent value and moisture content, which were 23.79 and 7.63%, respectively. The animals were divided into six groups, each five rats a group, namely, carrier, untreated, and positive control (levamisole), and TS at doses of 200, 400, and 800 mg/kg BW orally. Meanwhile, all animals except the carrier control group received CYD induction at a dose of 100 mg/kg BW on days 16 and 18. The investigated parameters, such as leukocytes and thrombocyte value, body weight, and nitric oxide (NO) secretion of the peritoneal macrophage were examined after 56 days of treatment. The results showed that there were no significant differences in the parameters of leukocyte and and thrombocyte value between the groups (p > 0.05). However, there was a significant increase in nitric oxide (NO) secretion at the dose of 200 mg/kg BW compared to the other groups (p < 0.05). This increase in NO production could be associated to a rise in the non-specific immune response. Therefore, it can be concluded that TS treatment at a dose of 200 mg/kg BW showed an immunostimulant effect on the CYD-induced rat model via nitric oxide production pathway. Nevertheless, further studies are needed to confirm the immunostimulant mechanism on other pathways.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Tiya N. Renggani
AU  - Idah Rosidah
AU  - Shelvi Listiana
AU  - Nizar
AU  - Kurnia Agustini
AU  - Sri Ningsih
PY  - 2023
DA  - 2023/03/01
TI  - The Nitric Oxide-Induced Immune Effect After Temulawak (Curcuma Xanthorrhizae Roxb.) Starch Treatment on Cyclophosphamide-Induced Rats
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 729
EP  - 739
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_66
DO  - 10.2991/978-94-6463-112-8_66
ID  - Renggani2023
ER  -