The Effect of Indonesian Royal Jelly Supplementation on the Growth of Hybridoma and Its Monoclonal Antibody Production

DOI

10.2991/978-94-6463-112-8_21How to use a DOI?

Keywords

culture medium; hybridoma; monoclonal antibody; proliferation; royal jelly

Abstract

Monoclonal antibody (mAb) is the material that is used widely for biological experiments, therapeutic, and diagnosis. The production of monoclonal antibodies by in vitro method is still challenging, and it takes a high cost due to the use of animal-derived serum as the main growth-promoting factor. The previous study reported royal jelly (RJ) from Apis mellifera as a potential proliferation factor in mammalian cell culture. Hence, this study was performed to evaluate the effect of Indonesian RJ supplementation in culture medium on hybridoma growth and its MAb production. The research was conducted by true experimental methods using hybridoma clone SF2 and RF10. These clones were cultured in RPMI-1640 medium supplemented with 3% FBS and 1% Penicillin-Streptomycin as the initial culture medium. The number of viable cells was counted by haemocytometer on day 8 after gradual treatment with or without the addition of 1% RJ. MTT assay was performed to evaluate the cell viability. In addition, the level of secreted MAb in the culture medium was measured by indirect enzyme-linked immunosorbent assay (ELISA). All results were analyzed statistically. The results show that the addition of 1% RJ into the culture medium has decreased the cell viability compared to the control. Thus, cell viability reduction may influence the level of MAb produced by hybridoma clone SF2 and RF10. The negative result given in this experiment may have been caused by an unsuitable concentration of RJ used in this experiment. Also, a substance included in the crude RJ probably causes cytotoxicity in the cells.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Febby N. Ningsih
AU  - Tika Widayanti
AU  - Muhamad Sahlan
AU  - Andri P. Pramono
AU  - Sabar Pambudi
PY  - 2023
DA  - 2023/03/01
TI  - The Effect of Indonesian Royal Jelly Supplementation on the Growth of Hybridoma and Its Monoclonal Antibody Production
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 210
EP  - 219
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_21
DO  - 10.2991/978-94-6463-112-8_21
ID  - Ningsih2023
ER  -