High Glucose Inhibits the Expression of EtcABC Which Resulted in Negative Regulation of Type III Fimbriae in Klebsiella pneumoniae Isolates

DOI

10.2991/978-94-6463-112-8_13How to use a DOI?

Keywords

biofilm formation; bacterial type III fimbriae; MrkA protein; virulence factor

Abstract

Klebsiella pneumoniae is one of abundant normal flora in human, but also well-studied as an opportunistic pathogen due to its ability to form biofilm. Biofilm could be formed by biofilm-forming bacteria as its survival method. Valid example for biofilm-associated medical issue is catheter-related diseases, such as urinary tract infection (UTI). In order to form the biofilm, bacteria utilize the fimbriae in order to form the attachment of bacterial cell and surface of medical equipments. Klebsiella pneumoniae has two types of fimbriae, type I and type III, which play big role in regulating its biofilm formation. We previously found that the expression of type III fimbriae and biofilm formation in K. pneumoniae strain STU1 were tightly regulated by cAMP-CRP pathway, and significantly increased by the overexpression of EtcABC, the component of bacterial phosphoenolpyruvate (PEP):carbohydrate phosphotransferase systems (PTS). Interestingly, by RT-qPCR and Western Blotting, we found that the expression of EtcA in STU1 (the homologue of KPN00353 in K. pneumoniae strain MGH78578) was reduced significantly by high glucose. The reduction of EtcA which was predicted to have role as an EIIA in PTS, significantly reduced the expression of MrkA, the main protein for type III fimbriae assembly and formation. We therefore took K. pneumoniae isolates isolated from Tzu Chi hospital in Hualien, Taiwan to follow the observation we found in STU1 and found the same pattern that the higher the concentration of glucose added in the bacterial culture medium, the less the expression of EtcA and MrkA. In diabetic mellitus patients, K. pneumoniae was often to be the causing agents of pyogenic liver abscess (PLA). From this point of view, we found interesting scenario that probably could be the mechanism of K. pneumoniae infection in diabetic mellitus patients. Our in vitro data strongly suggested that high glucose levels negatively affected the type III fimbriae expression and biofilm formation in K. pneumoniae STU1 and clinical isolates. Further study is indeed worth to be done for unraveling the unknown molecular mechanisms of Klebsiella biofilm formation and infections in certain type of patient conditions.

Copyright

© 2023 The Author(s)

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TY  - CONF
AU  - Novaria S. D. Panjaitan
AU  - Soo Po-Chi
AU  - Lin Yin-Jia
AU  - Tsai Yi-Jhen
PY  - 2023
DA  - 2023/03/01
TI  - High Glucose Inhibits the Expression of EtcABC Which Resulted in Negative Regulation of Type III Fimbriae in Klebsiella pneumoniae Isolates
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 125
EP  - 135
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_13
DO  - 10.2991/978-94-6463-112-8_13
ID  - Panjaitan2023
ER  -