Effect of Tween 20 Inclusion in Oligopeptide-Based Liposome Formulation on Transfection Agent/DNA Particle Size, Complex Stability and Gene Expression

DOI

10.2991/978-94-6463-112-8_18How to use a DOI?

Keywords

Tween 20; liposome formulation; transfection agent; particle size; nanoparticle

Abstract

The main requirement of a transfection agent has to condense DNA efficiently, protect the DNA from nucleases and other degrading enzymes during its transport in a target cell. In this research, Polysorbate 20 which is commercially known as Tween 20 has been supplemented to oligopeptide-based liposome formulation in order to achieve a stable and effective transfection agent in mediating gene expression. Complex particle size and its stability of transfection agent/DNA were determined by dynamic light scattering analysis. Transfection study was carried out using green fluorescence protein (GFP) encoding gene on HepG2 cells. It was revealed that the addition of Tween 20 (0.5% v/v) on liposome formulation reduced the particle size of the liposome/DNA complexes. The addition of Tween 20 in liposome capable to condense DNA molecules effectively. The particle size of the liposome/DNA at charge ratio 1.0 was ~340 nm; meanwhile, Tween 20 increased the zeta potential value at any charge ratio applied. Interestingly, the inclusion of the Tween 20 has also stabilized the liposome as the particle size almost unchanged after the complexes of the liposome/DNA was monitored for 240 h (10 days). Unfortunately, we did not observe that the addition of Tween 20 in the liposome formulation (0.5% v/v) has beneficial for gene expression as the GFP expression level reduced compared to the corresponding oligopeptide. In summary, Tween 20 (0.5% v/v) addition in the liposome formulation has condensed and stabilized the liposome/DNA complex in nanoparticle size, however, it needs further exploration in formulating Tween 20-containing liposome to achieve optimum beneficial in gene expression.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

TY  - CONF
AU  - Tarwadi Tarwadi
AU  - Damai R. Setyawati
AU  - Sabar Pambudi
AU  - Prasetyawan Yunianto
AU  - Rahmana E. Kartasasmita
AU  - Heni Rachmawati
AU  - Sukmadjaja Asyarie
PY  - 2023
DA  - 2023/03/01
TI  - Effect of Tween 20 Inclusion in Oligopeptide-Based Liposome Formulation on Transfection Agent/DNA Particle Size, Complex Stability and Gene Expression
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 180
EP  - 191
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_18
DO  - 10.2991/978-94-6463-112-8_18
ID  - Tarwadi2023
ER  -