Identification of Vibrio parahaemolyticus Strains by means of HPLC fingerprinting

DOI

10.2991/icmemtc-16.2016.18How to use a DOI?

Keywords

Vibrio parahaemolyticus; identification; high performance liquid chromatography

Abstract

The objective of this study was to identify Vibrio parahaemolyticus by HPLC, a simple, rapid, and reliable HPLC method. For the HPLC methods, an established for the fingerprints of Vibrio parahaemolyticus was performed on Strict extraction and reversed-phase HPLC conditions. An Agilent C18(4. 6 mm ×25 mm, 5 m) as column and acetonitrile-0.05% phosphoric acid solution in gradient as mobile phase. The detection wavelength was set at 280 nm and column temperature was 30°C. Established HPLC fingerprint was distinct and reproducible. There were 2 characteristic peaks. The HPLC fingerprints of Vibrio parahaemolyticus is characteristic and specific, and has the potential to replace biochemical methods because of its simplicity, rapidity, specificity, and cost-effectiveness. It is apparent from this study that we had developed a new, steady and rapid method for identification of Vibrio parahaemolyticus Strains. For the rapid identification of foodborne pathogens build a technology platform.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yiling Tian
AU  - Yunzhe Zhang
AU  - Hui Shi
AU  - Wei Zhang
PY  - 2016/04
DA  - 2016/04
TI  - Identification of Vibrio parahaemolyticus Strains by means of HPLC fingerprinting
BT  - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
PB  - Atlantis Press
SP  - 96
EP  - 101
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmemtc-16.2016.18
DO  - 10.2991/icmemtc-16.2016.18
ID  - Tian2016/04
ER  -