Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control

Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay

Authors
Zhenzhen Chen, Lixin Zhu, Renrong Liu, Wei Meng, Na Wu, Fanfan Yang, Kaihong Li, Yifan Lang
Corresponding Author
Zhenzhen Chen
Available Online April 2016.
DOI
10.2991/icmemtc-16.2016.19How to use a DOI?
Keywords
Deoxynivalenol; Chemiluminescence enzyme immunoassay; grain sorghum
Abstract

A chemiluminescence enzyme-linked immunosorbent assay (CLEIA) with enhanced chemiluminescent was developed for the detection of deoxynivalenol (DON) in grain sorghum. Assay conditions, including concentrations of antibody and enzyme conjugate, Na+ (NaCl), organic solvent (methyl alcohol) and pH were investigated. The optimized CLEIA allowed the DON detection in a linear working range of 0.588-26.783 ng mL-1 with the IC50 value of 3.968ng mL-1 and a limit of detection (LOD) of 0.311 ng mL-1. The CLEIA was one time more sensitive than the colorimetric ELISA by using the same antibody and HRP-conjugate. A series of spiked grain sorghum were detected by the method. The recovery rate ranged from 71.68% -96.56%, and the coefficient variation (CV) ranged from 5.93% -13%.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
Series
Advances in Engineering Research
Publication Date
April 2016
ISBN
978-94-6252-173-5
ISSN
2352-5401
DOI
10.2991/icmemtc-16.2016.19How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Zhenzhen Chen
AU  - Lixin Zhu
AU  - Renrong Liu
AU  - Wei Meng
AU  - Na Wu
AU  - Fanfan Yang
AU  - Kaihong Li
AU  - Yifan Lang
PY  - 2016/04
DA  - 2016/04
TI  - Determination of deoxynivalenol in grain sorghum by chemiluminescence enzyme immunoassay
BT  - Proceedings of the 2016 3rd International Conference on Materials Engineering, Manufacturing Technology and Control
PB  - Atlantis Press
SP  - 102
EP  - 105
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmemtc-16.2016.19
DO  - 10.2991/icmemtc-16.2016.19
ID  - Chen2016/04
ER  -