A Study on the Preparation and Characterization of Curcumin and Gold Nanorods-loaded Albumin Nanoparticles and its Treatment of MCF-7 Breast Cancer Cells in Vitro

DOI

10.2991/icmsa-15.2015.71How to use a DOI?

Keywords

Curcumin and Gold Nanorods-loaded Albumin Nanoparticles, Photothermal Therapy, Breast Cancer.

Abstract

This work aims to study the preparation and characterization of curcumin and gold nanorods-loaded albumin nanoparticles and investigate its treatment for MCF-7 human breast cancer cells in vitro. Gold nanorods (GNRs) were prepared by seedless method. While the curcumin and gold nanorods-loaded albumin nanoparticles (Cur/GNRs-BSA-NPs) were prepared by desolvation-crosslinking method, with the characteristics detected by dynamic light scattering (DLS), transmission electron microscope (TEM) and ultraviolet photometer. The results indicated that the GNRs and Cur/GNRs-BSA-NPs were prepared successfully. The prepared GNRs, which are rectangular and round-ended, have uniform sizes and good dispersion. The Cur/GNRs-BSA nanoparticles were spherical with exact average diameter of 190nm. The Cur/GNRs-BSA-NPs did not show cytotoxicity in vitro after tested by the CCK-8 assay. Thermodynamic test was employed to observe the temperature change of various doses of Cur/GNRs-BSA-NPs at the near-infrared (NIR) light exposure with the wavelength of 808 nm and the output power of 2.5 W. It was found that the Cur/GNRs-BSA-NPs induced significant temperature elevation and curcumin release upon irradiation with NIR light. The anti-tumor effect on MCF-7 human cancer cells treated with Cur/GNRs-BSA-NPs in vitro was evaluated by CCK-8 assay. The experimental results revealed that Cur/GNRs-BSA-NPs could significantly inhibit the proliferation of MCF-7 cells with combined treatment. Moreover, the coating of albumin could help reduce toxicity of GNRs and improve the bioavailability of the insoluble curcumin. The Cur/GNRs-BSA-NPs developed in this work present a new potential method combining both traditional Chinese and western medical treatment for human breast cancer.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chen Liang
AU  - Hao Zhang
AU  - Lin Wang
AU  - Dong-Sheng Zhang
PY  - 2014/06
DA  - 2014/06
TI  - A Study on the Preparation and Characterization of Curcumin and Gold Nanorods-loaded Albumin Nanoparticles and its Treatment of MCF-7 Breast Cancer Cells in Vitro
BT  - Proceedings of the 2015 International Conference on Material Science and Applications
PB  - Atlantis Press
SP  - 388
EP  - 394
SN  - 2352-541X
UR  - https://doi.org/10.2991/icmsa-15.2015.71
DO  - 10.2991/icmsa-15.2015.71
ID  - Liang2014/06
ER  -