Proceedings of the First International Conference on Health, Social Sciences and Technology (ICoHSST 2020)

How to Extract and to Examination of β-Carotene in Carrot (Daucus carota)

Authors
Muhamad Taswin, Sonlimar Mangunsong
Corresponding Author
Muhamad Taswin
Available Online 19 April 2021.
DOI
https://doi.org/10.2991/assehr.k.210415.052How to use a DOI?
Keywords
β-carotene, Carrot, HPLC, GC-MS, Carotenoid
Abstract
Carrots (Daucus carota), is the main sources of beta carotene besides tomatoes and palm oil. At present, β-carotene has been used extensively in food, industry, and medicine, so that beta carotene is of high economic value. The β-carotene in carrot can be obtained by chemical preparation. The researchers, usually to have β- carotene mainly done with organic solvents. It is very rarely done without a chemical solvent. This study aims to extract β-carotene without chemical solvent and also to determine β- carotene using UHPLC and GC-MS. Approximately 300 gram carrots, cleaned, cut into small pieces and mashed in a blender, given aqua 20 mL. separate the filtrate from that matrix. To the filtrate was then, added some chemical salt, centrifuged 5000 x g for 30 minutes to have pellet. The pellets are then removed, evaporated with the vacuum to dry at a temperature of 40o C for 48 hours. β-carotene pellets are measured with UV-vis, HPLC and GC-MS. Using column UHPLC and the mobile phase was chloroform-methanol (95: 5) with a flow rate of 1 ml /minute at a UV detector wavelength of 460 nm. The retention time examination of β-carotene and standard (sigma beta carotene) using HPLC and GC-MS was 3.75 and 9,80 minutes respectively. GC-MS examination showed ions and molecules of carotenoid. Research showed that this method can be used to be developed for the withdrawal of β-carotene in carrots and others, without any need for organic solvents.
Open Access
This is an open access article distributed under the CC BY-NC license.

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Cite this article

TY  - CONF
AU  - Muhamad Taswin
AU  - Sonlimar Mangunsong
PY  - 2021
DA  - 2021/04/19
TI  - How to Extract and to Examination of β-Carotene in Carrot (Daucus carota)
BT  - Proceedings of the First International Conference on Health, Social Sciences and Technology (ICoHSST 2020)
PB  - Atlantis Press
SP  - 252
EP  - 256
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210415.052
DO  - https://doi.org/10.2991/assehr.k.210415.052
ID  - Taswin2021
ER  -