Proceedings of the First International Conference on Health, Social Sciences and Technology (ICoHSST 2020)

How to Extract and to Examination of β-Carotene in Carrot (Daucus carota)

Authors
Muhamad Taswin, Sonlimar Mangunsong
Corresponding Author
Muhamad Taswin
Available Online 19 April 2021.
DOI
10.2991/assehr.k.210415.052How to use a DOI?
Keywords
β-carotene, Carrot, HPLC, GC-MS, Carotenoid
Abstract

Carrots (Daucus carota), is the main sources of beta carotene besides tomatoes and palm oil. At present, β-carotene has been used extensively in food, industry, and medicine, so that beta carotene is of high economic value. The β-carotene in carrot can be obtained by chemical preparation. The researchers, usually to have β- carotene mainly done with organic solvents. It is very rarely done without a chemical solvent. This study aims to extract β-carotene without chemical solvent and also to determine β- carotene using UHPLC and GC-MS. Approximately 300 gram carrots, cleaned, cut into small pieces and mashed in a blender, given aqua 20 mL. separate the filtrate from that matrix. To the filtrate was then, added some chemical salt, centrifuged 5000 x g for 30 minutes to have pellet. The pellets are then removed, evaporated with the vacuum to dry at a temperature of 40o C for 48 hours. β-carotene pellets are measured with UV-vis, HPLC and GC-MS. Using column UHPLC and the mobile phase was chloroform-methanol (95: 5) with a flow rate of 1 ml /minute at a UV detector wavelength of 460 nm. The retention time examination of β-carotene and standard (sigma beta carotene) using HPLC and GC-MS was 3.75 and 9,80 minutes respectively. GC-MS examination showed ions and molecules of carotenoid. Research showed that this method can be used to be developed for the withdrawal of β-carotene in carrots and others, without any need for organic solvents.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the First International Conference on Health, Social Sciences and Technology (ICoHSST 2020)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
19 April 2021
ISBN
10.2991/assehr.k.210415.052
ISSN
2352-5398
DOI
10.2991/assehr.k.210415.052How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Muhamad Taswin
AU  - Sonlimar Mangunsong
PY  - 2021
DA  - 2021/04/19
TI  - How to Extract and to Examination of β-Carotene in Carrot (Daucus carota)
BT  - Proceedings of the First International Conference on Health, Social Sciences and Technology (ICoHSST 2020)
PB  - Atlantis Press
SP  - 252
EP  - 256
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210415.052
DO  - 10.2991/assehr.k.210415.052
ID  - Taswin2021
ER  -