Proceedings of the 4th International Conference on Sustainable Innovation 2020–Technology, Engineering and Agriculture (ICoSITEA 2020)

Evaluation of Antagonistic Activity of Trichoderma harzianum Against Various Pathogenic Fungi Infecting Dragon Fruit Stem

Authors
Agung Astuti, Salmaa Muftiyatunnisa, Dina Wahyu Trisnawati
Corresponding Author
Agung Astuti
Available Online 10 February 2021.
DOI
https://doi.org/10.2991/aer.k.210204.040How to use a DOI?
Keywords
Dragon fruit, Trichoderma harzianum, Phytium sp, Fusarium sp, Alternaria sp, Rhizoctonia sp
Abstract

Dragon fruit stem is prone to the infection of various pathogenic fungi and Trichoderma harzianum had been reported as an effective biological control agent against plant pathogenic fungi. This study was aimed to evaluate the ability of T. harzianum in controlling various pathogenic fungi infecting dragon fruit stem. This study was conducted using Completely Randomized Design (CRD) with three replicates. Pathogenic fungi was isolated from infected dragon fruit stem, then characterized morphologically to identify the species of those pathogenic fungi. The isolated fungi were subjected to in-vitro and in-planta antagonistic assay containing T. harzianum culture. The resulted growth inhibition in each fungal isolate was observed for a week by comparing the diameter of pathogenic fungi. Based on morphological characteristics, the dragon fruit stem was predicted to be infected by several pathogenic fungi, such as Phytium sp. (isolate A), Fusarium sp. (isolate B), Alternaria sp. (isolate C) and Rhizoctonia sp. (isolate D). Result of in-vitro antagonistic assay showed that T. harzianum conferred different suppression efficacy against all isolated pathogenic fungi ranging from 59-66%. Moreover, application of T. harzianum in-planta had resulted promising fungal growth inhibition up to 50% against all pathogenic fungi.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 4th International Conference on Sustainable Innovation 2020–Technology, Engineering and Agriculture (ICoSITEA 2020)
Series
Advances in Engineering Research
Publication Date
10 February 2021
ISBN
978-94-6239-334-9
ISSN
2352-5401
DOI
https://doi.org/10.2991/aer.k.210204.040How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Agung Astuti
AU  - Salmaa Muftiyatunnisa
AU  - Dina Wahyu Trisnawati
PY  - 2021
DA  - 2021/02/10
TI  - Evaluation of Antagonistic Activity of Trichoderma harzianum Against Various Pathogenic Fungi Infecting Dragon Fruit Stem
BT  - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Technology, Engineering and Agriculture (ICoSITEA 2020)
PB  - Atlantis Press
SP  - 218
EP  - 221
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.210204.040
DO  - https://doi.org/10.2991/aer.k.210204.040
ID  - Astuti2021
ER  -