Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene
Authors
Wimbuh Tri Widodo, Abdul Hadi furqoni, Ahmad Yudianto, Sri Puji Astuti Wahyuningsih
Corresponding Author
Wimbuh Tri Widodo
Available Online August 2017.
- DOI
- 10.2991/icpsuas-17.2018.81How to use a DOI?
- Keywords
- Polimerase Chain Reaction (PCR); DNA; human and Animal Blood Mixtures; human cytochrome b
- Abstract
Human and animal bloods expecially vertebrata bloods have similar composition so it were difficult to distinguish. The aim of this study was to identify human blood from human and animal blood mixtures using cytochrome b gene that has the species-specific DNA sequence. DNA extracted from variated human and chicken bloods were amplified using human-specific cytochrome b gene primer. The result showed 10 % human blood from mixtures could be identified and DNA extracted from human bloods only could be amplified. The concentration of 0.01 ng still showed DNA band. This primer was very sensitive and specific so it useful for forensic purposes to verify human blood in a blood mixture.
- Copyright
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Wimbuh Tri Widodo AU - Abdul Hadi furqoni AU - Ahmad Yudianto AU - Sri Puji Astuti Wahyuningsih PY - 2017/08 DA - 2017/08 TI - Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene BT - Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017) PB - Atlantis Press SP - 371 EP - 373 SN - 2352-5398 UR - https://doi.org/10.2991/icpsuas-17.2018.81 DO - 10.2991/icpsuas-17.2018.81 ID - TriWidodo2017/08 ER -