Proceedings of the International Conference on Research and Academic Community Services (ICRACOS 2019)

In Vitro Mucolytic Activity Assay of The Acetone Extract of The Silver Fern (Pityrogramma calomelanos)

Authors
Suyatno Sutoyo, Tukiran, Nur Indah Kumala Sari
Corresponding Author
Suyatno Sutoyo
Available Online January 2020.
DOI
https://doi.org/10.2991/icracos-19.2020.29How to use a DOI?
Keywords
Pityrogramma calomelanos, acetone extract, mucolyic activity
Abstract

The aims of this research is to determine mucolytic activity of methanol extract of silver fern (P. calomelanos). In this research extraction was carried out by maceration method. Qualitative test was done by ferric chloride reagent and Shinoda test to identify the existance of phenolic and flavonoid compounds, respectively. Mucolytic activity assay was performed in vitro based on the decreasing the viscosity of intestinal mucus cow. Viscosity values ​​were analyzed statistically using one-way ANOVA test, followed by LSD test to determine significant differences between treatment groups. From the results of the study, it had been obtained the methanol extract in the form of brown solid. The qualitative test of the extract with the ferric chloride reagent produced a green color which showed the existence of phenolic compound, while the orange color in the Shinoda Test showed the existence of flavonoid compound. The result of mucolytic activity test showed that the extract had mucolytic activity. Extract with a concentration of 0.6% had mucolytic activity equivalent to acetylcysteine ​​0.1%.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Conference on Research and Academic Community Services (ICRACOS 2019)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
January 2020
ISBN
978-94-6252-881-9
ISSN
2352-5398
DOI
https://doi.org/10.2991/icracos-19.2020.29How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Suyatno Sutoyo
AU  - Tukiran
AU  - Nur Indah Kumala Sari
PY  - 2020/01
DA  - 2020/01
TI  - In Vitro Mucolytic Activity Assay of The Acetone Extract of  The Silver Fern (Pityrogramma calomelanos)
BT  - Proceedings of the International Conference on Research and Academic Community Services (ICRACOS 2019)
PB  - Atlantis Press
SP  - 137
EP  - 140
SN  - 2352-5398
UR  - https://doi.org/10.2991/icracos-19.2020.29
DO  - https://doi.org/10.2991/icracos-19.2020.29
ID  - Sutoyo2020/01
ER  -