Molecular Characterization of Riau’s Mascot Flora (Oncosperma tigillarium)
- DOI
- 10.2991/assehr.k.210305.004How to use a DOI?
- Keywords
- DNA isolation, Oncosperma tigillarium, Doyle and Doyle method
- Abstract
This research was aims to find out about the molecular characterization of the Nibung plant (Oncosperma tigillarium). It has been conducted in the Biology Laboratory. The results of DNA isolation were tested to determine the quality of DNA that has been isolated. DNA purity was determined based on A260/A280 comparison values were tested using a nanodrop spectrophotometer (Sambrook et al. (1989) method. The quality of DNA from isolation was influenced by several factors such as the condition of the leaf sample used, the length of time of grinding, the phenolic content of the sample, the process of taking the supernatant solution and others. In this study, these factors were seen to significantly affect the quality of the DNA isolated. Also, DNA fragments generated from the isolation process are large, which indicates the large concentration of DNA produced. Although many DNA bands smear they still have clear DNA bands. Overall, the DNA isolation method based on the Doyle Doyle (1987) method in this study is applied to Nibung plants which is used as Riau’s Province mascot flora.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Desti AU - Fitmawati AU - Putri Ade Rahma Yulis AU - Mayta Novaliza Isda PY - 2021 DA - 2021/03/08 TI - Molecular Characterization of Riau’s Mascot Flora (Oncosperma tigillarium) BT - Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020) PB - Atlantis Press SP - 23 EP - 27 SN - 2352-5398 UR - https://doi.org/10.2991/assehr.k.210305.004 DO - 10.2991/assehr.k.210305.004 ID - 2021 ER -