Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)

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Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker

Authors
Fay Della Prika Auvira, Ixora Sartika Mercuriani, Suyitno Aloysius
Corresponding Author
Fay Della Prika Auvira
Available Online 8 March 2021.
DOI
10.2991/assehr.k.210305.011How to use a DOI?
Keywords
Spathoglottis plicata, Genetic diversity, PCR-RAPD
Abstract

The purpose to know the genetic variability of orchid variants Spathoglottis plicata based on molecular marker Random Amplified Polymorphic DNA (RAPD). DNA isolation carried out using TIANGEN Plant Genomic DNA kit. DNA sample was amplified using PCR-RAPD technique. Genetic variability were analyzed using GenAlex 6.1 software to obtain the percentage of polymorphism, genetic distance and principal coordinates. Moreover, NTSYS Version 2.02 Unweighted Pair-Group Method using Arithmetic Average (UPGAMA) was used to create cluster dendrogram based on similarity indeces of S. plicata orchid variants. The result showed that DNA polymorphism of S. plicata orchid variants is 53,28%. Based on the Principal Coordinate Analysis (PCA), orchid variants S. plicata are spread in four quadrants in which the mutant variants on the opposite quadrant with wild type plants. Based on dendrogram, all orchid variants S. plicata are devided into 2 clusters of mutant plants which has a lower similarity index (<0,64) compared to wild type (>70) showing that mutant variants are potential to be new cultivars.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
8 March 2021
ISBN
10.2991/assehr.k.210305.011
ISSN
2352-5398
DOI
10.2991/assehr.k.210305.011How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Fay Della Prika Auvira
AU  - Ixora Sartika Mercuriani
AU  - Suyitno Aloysius
PY  - 2021
DA  - 2021/03/08
TI  - Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker
BT  - Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)
PB  - Atlantis Press
SP  - 70
EP  - 75
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.210305.011
DO  - 10.2991/assehr.k.210305.011
ID  - Auvira2021
ER  -