Molecular Characterization of Orchid Variants Spathoglottis plicata Blume Based on RAPD Analysis
- 10.2991/assehr.k.210305.009How to use a DOI?
- Spathoglottis plicata variants, genetic diversity, RAPD
The development of S. plicata orchid variants is important to increase their genetic diversity. This study aims to determine the genetic diversity of S. plicata orchid variants based on RAPD analysis. The research sample was 15 plant variants consisting of wild type and mutant orchid variants. Molecular characterization was used to identify the genetic diversity of orchid variants based on the PCR-RAPD using eight universal primers from the screening results. The DNA amplicon electropherogram from RAPD results was converted to binary data and analyzed with GenAlex 6.1 to obtain the genetic distance, scatter diagram, and the percentage of DNA polymorphism. The data were also analyzed using NTSYS ver.2.2 to obtain a dendrogram cluster using the Unweighted Pair-Group Method using Arithmetic Average (UPGMA). The results showed that DNA polymorphism was found in the wild-type and mutant orchid variants. The percentage of DNA polymorphism of mutant orchid groups was greater than that of wild type orchids. The results of the Principal Coordinate Analysis based on the genetic distance showed that the S. plicata mutant orchid variant was in a different quadrant from the wild type group. Based on the dendrogram cluster, the mutant orchid variants were located away from the wild orchid, which confirmed that the mutant orchid variants have undergone genetic changes.
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Suyitno Aloysius AU - Ixora S. Mercuriani AU - Ratnawati AU - Sudarsono PY - 2021 DA - 2021/03/08 TI - Molecular Characterization of Orchid Variants Spathoglottis plicata Blume Based on RAPD Analysis BT - Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020) PB - Atlantis Press SP - 56 EP - 61 SN - 2352-5398 UR - https://doi.org/10.2991/assehr.k.210305.009 DO - 10.2991/assehr.k.210305.009 ID - Aloysius2021 ER -